Establishment of a Novel Model for Hypothermic Machine Perfusion In Vivo in Rabbits

Z. Zhong,¹ Z. Liu,¹ R. Wang,¹ Z. Fu,² Y. Zhang,² Q. Hu,¹ K. Jin,¹ D. Ko,³ Y. Wang,¹ Q. Ye.^1,2

¹Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei, China
²The 3rd Xiangya Hospital of Central South University, Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology, Changsha, Hunan, China
³Massachusetts General Hospital, Department of Urology, Boston, MA.

Meeting: 2015 American Transplant Congress

Abstract number: B92

Keywords: Donors, Machine preservation, non-heart-beating, Survival, Warm ischemia

Session Information

Session Name: Poster Session B: Kidney and Donor Evaluation/Utilization

Session Type: Poster Session

Date: Sunday, May 3, 2015

Session Time: 5:30pm-6:30pm

Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

Background: Hypothermic machine perfusion (MP) could improve outcome after transplantation of DCD kidneys, but protective mechanisms of MP remain largely unknown. Therefore, this study aims to establish a novel animal model and lay a foundation for mechanism of MP.

Methods: The same quality male rabbits were randomly divided into MP and CS groups (n=18). Each group was further divided into group MP25min, MP35min, MP45min and CS25min, CS35min, CS45min. The left renal pedicles of rabbits were clamped for 25 min, 35 min and 45 min at 38.5±0.2°C, followed by reperfusion for 1 h. Left kidneys were preserved by MP (LifePort Kidney Transporter machines) or by CS in vivo for 4 h, then blood vessel were sutured and right kidneys were resected, the specimens were collected (n=6 per group) after 24 hours' reperfusion.

Results: There were no statistical differences in survive rates in group MP25min and CS25min, in group MP45min and CS45min. The urine volume after 24 hours was 150 ± 10 ml in group MP25min, 50±10 ml in group CS25min, 110±20 ml in group MP35min, 35±8 ml in group CS35min (P< 0.05), 10±2 ml in group MP45min, 8±2 ml in group CS45min (P> 0.05). The creatinine after 24 hours was 300±14 μmol/l in group MP25min, 480±23μmol/l in group CS25min, 355±71μmol/l in group MP35min, 511±44μmol/l in group CS35min (P< 0.05). The rate of apoptosis was 1.32±0.004% in group MP25min, 8.18±0.021% in group CS25min, 4.31±0.008% in group MP35min, 16.81±0.014% in group CS35min (P< 0.05), 26.81±2.44% in group MP45min, 27.21±3.41% in group CS45min.

Conclusion: Hypothermic machine perfusion improves kidney viability in rabbits below 35 minutes' warm ischemia (WI), which is invalid after 45 minutes' warm ischemia.

To cite this abstract in AMA style:

Zhong Z, Liu Z, Wang R, Fu Z, Zhang Y, Hu Q, Jin K, Ko D, Wang Y, Ye Q. Establishment of a Novel Model for Hypothermic Machine Perfusion In Vivo in Rabbits [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/establishment-of-a-novel-model-for-hypothermic-machine-perfusion-in-vivo-in-rabbits/. Accessed May 17, 2025.

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