ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Effect of Sirtuin 1 Gene Expression on Mouse Islet Graft Survival, The

Y. Kim, Y. Wee, M. Choi, J. Chang, C. Yun, D. Han

Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea
Division of Nephrology, Department of Internal Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea
Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea

Meeting: 2013 American Transplant Congress

Abstract number: A675

Related Abstracts
  • Survival of Free and Encapsulated Islet Xenografts Transplanted in the Mouse Bone Marrow, The
  • Knockdown of Intra-Islet IKKβ by Spherical Nucleic Acid Nanoparticle Conjugates Prevents Host Cytokine-Induced Injury and Enhances Islet Graft Survival

Purpose: Islet transplantation is a promising therapy for Type 1 diabetes, however clinical application has not been as successful as organ transplantation due to early graft injury as well as immune reaction. Sirtuin 1 (SIRT1), a nicotinamide adenosine dinucleotide-dependent histone deacetylase, has been shown to up-regulate insulin secretion in response to glucose stimulation in pancreatic Β-cells. SIRT1 gene transfection of islet before transplantation could ameliorate their capacity to survive and engraft in the implanted site.

Materials and Methods: In this study, we investigated whether SIRT1 gene could be transfected into islets by adenoviral vector and protect them from cytokine-mediated damage. We also examined the protective effect of SIRT1 transfected islets on graft failure after islet transplantation.

Results: We confirmed that adenoviral vector transfection of islets was efficient by quantitative analysis of GFP positive cell. In addition, SIRT1 expression was shown in transfected islets by Western blotting. Viability and insulin secretion were similar between transfected and control islets in normal condition. However in TNF-Α and IL-1Β treatment, significantly improved Ad SIRT1 transfected islet survival and function were detected compared with marked cell death in control islet. Furthermore, inhibition of SIRT1 activity by EX527 impaired viability and insulin secretion. We transplanted syngeneic islets (400 islet equivalents per recipient) with or without Ad-SIRT1 into the kidney capsule of diabetic C57BL/6 mice. Regarding blood glucose control, diabetic mice transplanted with a marginal mass of Ad-SIRT1 transfected islets became normoglycemic days more rapidly and 100% of the recipients were normoglycemic at 4.5 post-transplant day, whereas only 66% of the mice transplanted with Ad-LacZ transfected islets were normoglycemic at 18.5 post-transplant day (P<0.05).

Conclusion: These results suggested that delivery of the SIRT1 gene to islets enhanced islet cell survival during the early post-transplant period, and preserved islet mass and functions over time in the murine islet transplant model.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Kim Y, Wee Y, Choi M, Chang J, Yun C, Han D. Effect of Sirtuin 1 Gene Expression on Mouse Islet Graft Survival, The [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/effect-of-sirtuin-1-gene-expression-on-mouse-islet-graft-survival-the/. Accessed January 18, 2021.

« Back to 2013 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.