Mesenchymal stem cells (MSC) have proven immunomodulatory capacity (in vitro and in vivo) which makes them a promising therapeutic tool in transplantation. However, research on the immunosuppressive effects of MSC has mainly focused on T cell-mediated effector mechanisms and less is known about the effects of MSC on B cell-mediated immune responses. In the present study we investigated the immunomodulatory effect of MSCs on B cell differentiation and proliferation.
MSCs were isolated from subcutaneous fat tissue from kidney transplant donors obtained at the moment of the surgery.
B cells were obtained from human tonsils. After isolation of mononuclear cells by density gradient, resting mature B cells were obtained by CD43 negative selection with Magnetic Activated Cell Sorting (MACS).
MSC were co-cultured with CFSE labeled B cells stimulated in a T cell-dependent (anti-IgM + anti-CD40 + IL2) and T cell-independent (anti-IgM + IL2) manner.
At day 10 proliferation and B cell phenotype were analyzed by Flow Cytometry, and IgG production was measured in the supernatant by ELISA.
MSCs shifted the balance of naÏve (CD19+ CD27–)/memory (CD19+ CD27+) B cells stimulated in a T cell-independent manner towards the more naÏve B cells. Moreover, independently of the stimulation used, MSCs induced an increase in the percentage of CD19+ CD27– CD38high B cells, which include B cells with a regulatory phenotype.
MSC completely abolished the induction of CD19+ CD27high CD38high plasmablasts when the B cells were stimulated in a T cell-dependent manner and reduced the IgG production.
MSCs affect B cell differentiation, shifting the balance naÏve/memory B cells when stimulated in a T cell-independent manner. Furthermore, MSC reduce the differentiation of B cells to plasmablasts while increasing the percentage of regulatory-like B cells independently of the stimulation used. MSC might be important in regulating the humoral response in transplant rejection.
To cite this abstract in AMA style:Franquesa M, Mensah F, Huizinga R, Betjes M, Weimar W, Baan C, Hoogduin M. Effect of Adipose Tissue Derived Mesenchymal Stem Cells on B Cells Proliferation and Differentiation, The [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/effect-of-adipose-tissue-derived-mesenchymal-stem-cells-on-b-cells-proliferation-and-differentiation-the/. Accessed April 17, 2021.
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