Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis.

A. Yang, W. Ju, Z. Guo, C. Wu, J. Lin, M. Chen, R. Deng, E. Chen, Y. Gao, X. Zhu, X. He.

Organ Transplantation Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Meeting: 2016 American Transplant Congress

Abstract number: C285

Keywords: Genomic markers, Infection, Monitoring, Prediction models

Session Information

Session Name: Poster Session C: Viruses and SOT

Session Type: Poster Session

Date: Monday, June 13, 2016

Session Time: 6:00pm-7:00pm

Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Aims: Mortality of CMV infection in post-transplanted patients was quite high, which severely influencing the long-term survival of recipients. For giving them early treatment and improving their prognosis, we aimed to discover a novel diagnostic method to enhance the early diagnosis rate of CMV infection.

Methods: A murine model of CMV infection after skin transplantation was established.Peripheral blood was collected at a certain time point and screened by gene microarrays. Differentially expressed microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) were homologous analyzed with those of human and verified by qRT-PCR. Finally, we confirmed the potential biomarkers in the blood sample of post-transplanted patients.

Results: After gene chips screening, humanized comparation and qRT-PCR verification, differentially expressed 13 miRNAs and 7 lncRNAs in peripheral blood were discovered. Expression of miR-29b, miR-185 and NR_073415.2 in peripheral blood mononuclear cells were significantly decreased in patients with cytomegalovirus infection (P<0.001) during the early stage and kept downregulating for more than 21 days.

Conclusions: Circulating miR-29b, miR-185 and NR_073415.2 were decreased in post-transplanted patients with cytomegalovirus infection, and they may represent candidate biomarkers for early diagnosis.

CITATION INFORMATION: Yang A, Ju W, Guo Z, Wu C, Lin J, Chen M, Deng R, Chen E, Gao Y, Zhu X, He X. Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis. Am J Transplant. 2016;16 (suppl 3).

To cite this abstract in AMA style:

Yang A, Ju W, Guo Z, Wu C, Lin J, Chen M, Deng R, Chen E, Gao Y, Zhu X, He X. Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/down-regulated-of-circulating-mir-29b-mir-185-and-nr_073415-2-in-post-transplanted-patients-with-cytomegalovirus-infection-may-serve-as-novel-biomarkers-for-early-diagnosis/. Accessed July 9, 2025.

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