Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis.
Organ Transplantation Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Meeting: 2016 American Transplant Congress
Abstract number: C285
Keywords: Genomic markers, Infection, Monitoring, Prediction models
Session Information
Date: Monday, June 13, 2016
Session Name: Poster Session C: Viruses and SOT
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Aims: Mortality of CMV infection in post-transplanted patients was quite high, which severely influencing the long-term survival of recipients. For giving them early treatment and improving their prognosis, we aimed to discover a novel diagnostic method to enhance the early diagnosis rate of CMV infection.
Methods: A murine model of CMV infection after skin transplantation was established.Peripheral blood was collected at a certain time point and screened by gene microarrays. Differentially expressed microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) were homologous analyzed with those of human and verified by qRT-PCR. Finally, we confirmed the potential biomarkers in the blood sample of post-transplanted patients.
Results: After gene chips screening, humanized comparation and qRT-PCR verification, differentially expressed 13 miRNAs and 7 lncRNAs in peripheral blood were discovered. Expression of miR-29b, miR-185 and NR_073415.2 in peripheral blood mononuclear cells were significantly decreased in patients with cytomegalovirus infection (P<0.001) during the early stage and kept downregulating for more than 21 days.
Conclusions: Circulating miR-29b, miR-185 and NR_073415.2 were decreased in post-transplanted patients with cytomegalovirus infection, and they may represent candidate biomarkers for early diagnosis.
CITATION INFORMATION: Yang A, Ju W, Guo Z, Wu C, Lin J, Chen M, Deng R, Chen E, Gao Y, Zhu X, He X. Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Yang A, Ju W, Guo Z, Wu C, Lin J, Chen M, Deng R, Chen E, Gao Y, Zhu X, He X. Down-Regulated of Circulating miR-29b, miR-185 and NR_073415.2 in Post-Transplanted Patients with Cytomegalovirus Infection May Serve as Novel Biomarkers for Early Diagnosis. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/down-regulated-of-circulating-mir-29b-mir-185-and-nr_073415-2-in-post-transplanted-patients-with-cytomegalovirus-infection-may-serve-as-novel-biomarkers-for-early-diagnosis/. Accessed January 18, 2021.« Back to 2016 American Transplant Congress