ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Direct Alloimmunity Is Driven by Recipient APCs Displaying Donor MHC Molecules.

J. Marino,1 J. Paster,1 P. Crosby,1 M. Uehara,2 S. Mordecai,1 R. Abdi,2 G. Tocco,1 G. Benichou.1

1Surgery Department, Center for Transplantation Sciences, Massachusetts General Hospital/Harvard Medical School, Boston, MA
2Department of Medicine, Renal Division, Brigham and Women's Hospital, Boston, MA.

Meeting: 2016 American Transplant Congress

Abstract number: B13

Keywords: Allorecognition, MHC class I, MHC class II, Mice

Session Information

Session Name: Poster Session B: Allograft Rejection, Tolerance, and Xenotransplantation

Session Type: Poster Session

Date: Sunday, June 12, 2016

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

We have studied the trafficking of donor passenger leukocytes and donor MHC presentation in the lymphoid organs of mice having received an MHC-mismatched skin or heart transplant. After skin grafting, we observed a lack of migrating donor passenger leukocytes. On the contrary, high numbers of recipient dendritic cells and some B cells displaying donor MHC antigens on their surface. The majority of transferred allogeneic MHC molecules were MHC class II proteins carried by donor-derived vesicles secreted mainly but not exclusively by donor dendritic cells and B cells. Alternatively, in heart-transplanted mice, we found low but significant numbers of donor leukocytes (100/million cells) spread in draining, non-draining lymph nodes and the spleen. However, the frequency of these cells declined markedly from day 1 through 7 post-transplantation. Alternatively, we detected high numbers of recipient cells cross-dressed with donor MHC (>2000/million cells) in the spleen of recipients. This number gradually increased to reach over 15,000 cross-dressed cells/million spleen cells at day 10 post-transplantation. The vast majority (> 80%) of host cross-dressed cells and donor passenger leukocytes found in the spleen of heart-transplanted mice were CD20+ B cells, while no CD11c+ DCs were observed. Finally, we showed that recipient cells cross-dressed with donor MHC molecules could activate direct alloresponses in vitro and in vivo. Altogether, our study support the view that the presentation of donor MHC by host APCs in recipient lymphoid organs is the main mechanism underlying sensitization of alloreactive T cells after transplantation.

CITATION INFORMATION: Marino J, Paster J, Crosby P, Uehara M, Mordecai S, Abdi R, Tocco G, Benichou G. Direct Alloimmunity Is Driven by Recipient APCs Displaying Donor MHC Molecules. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Marino J, Paster J, Crosby P, Uehara M, Mordecai S, Abdi R, Tocco G, Benichou G. Direct Alloimmunity Is Driven by Recipient APCs Displaying Donor MHC Molecules. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/direct-alloimmunity-is-driven-by-recipient-apcs-displaying-donor-mhc-molecules/. Accessed June 1, 2025.

« Back to 2016 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences