ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Different Effects of mTOR Inhibitor VS CN Inhibitor on Human B Cells Proliferations and Activation Markers

G. La Monica, L. Xu, G. Chalasani, L. Gallon

Nephrology/Medicine, Northwestern University, Chicago, IL
Renal-Electrolyte/Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA

Meeting: 2013 American Transplant Congress

Abstract number: D1467

Related Abstracts
  • Comprehensive Analyses of Inhibitory Effects of the mTOR Inhibitor Everolimus on the Differentiation into Subsets of Human T-Helper Cells and B Cells
  • Differential Regulation of Regulatory T Cells and Th17 Cells by mTOR Inhibitor in Kidney Transplant Recipients

Background: Humoral immunity is increasingly recognized as an important factor in promoting transplant rejection. However, it is not well known how commonly used immunosuppressive drugs CN inhibitors (Tacrolimus, TAC) or mTOR inhibitors (Sirolimus, SRL) impact the differentiation and activation of subpopulations of human B cells.

Method: Peripheral blood mononuclear cells were isolated from healthy volunteers and CD19+ B cells were selected using CD19 magnetic microbeads and cultured in the presence of anti-IgM, anti-CD40, IL-21 (BCR/CD40-stimulation) for 6 days. A variety of parameters of B cell activity including proliferation, activation, differentiation and cytokine productions were monitored by flow cytometry.

Results: SRL at clinically relevant concentrations (e.g. 6 ng/ml) profoundly inhibited CD19+ B cell proliferation (up to ∼2 folds) compared to controls whereas TAC at 6-10 ng/ml had a minimal effect (Fig. 1). SRL, but not TAC inhibits the proliferation of CD19+CD27- naÏve and CD19+CD27+ memory B cells.

SRL effectively blocked plasma cell differentiation (% of CD19+CD138+) even at low dose (e.g. 2 ng/ml), and totally eliminated this cell population at over 6 ng/ml (% of CD19+ CD138+ and % of Blimp1+/Pax5 (low) transcriptional factors), but both SRL and TAC decreased % of CD24hiCD38hi Breg subset without affecting CD21hiCD23hi Breg subset. B cells cultured in the presence of SRL result in a population shift toward an activated phenotype (CD25+/CD69+) which resembles follicular B cells (CD21-CD23+IgM+IgD+).

Conclusions: The data show that SRL and TAC have different effects on B cells proliferation and differentiation. This study can help to define the use of immunosuppressive drugs in humoral alloimmune responces in transplantation.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Monica GLa, Xu L, Chalasani G, Gallon L. Different Effects of mTOR Inhibitor VS CN Inhibitor on Human B Cells Proliferations and Activation Markers [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/different-effects-of-mtor-inhibitor-vs-cn-inhibitor-on-human-b-cells-proliferations-and-activation-markers/. Accessed December 13, 2019.

« Back to 2013 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Home
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Penis Transplantation: First U.S. Experience.
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Tacrolimus Dose and Trough Blood Levels in a US Liver Transplant Population
  • Penis Transplantation: First U.S. Experience.

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2019 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

wiley-new-logo

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.