ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Comprehensive Functional Assessment of the Pathogenic Potential of Immunodominant Donor Specific Antibody Following Treatment of Late Antibody Mediated Rejection or Mixed Acute Rejection.

A. Lichvar,1 E. Portwood,1 P. Brailey,1 S. Tremblay,1 A. Shields,2 B. Abu Jawdeh,1 J. Kremer,2 F. Paterno,1 R. Alloway,1 A. Girnita,1 E. Woodle.1

1U of Cincinnati, Cincinnati
2Christ Hospital, Cincinnati

Meeting: 2017 American Transplant Congress

Abstract number: 303

Keywords: Antibodies

Session Information

Date: Monday, May 1, 2017

Session Name: Concurrent Session: B Cells and Antibody in Rejection

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:30pm-4:42pm

Location: E350

Related Abstracts
  • Early and Late Antibody Mediated Rejection and Mixed Acute Rejection Are Clinically Distinct and Demonstrate Biologic Differences in Immunodominant Donor Specific Antibody Characteristics.
  • Immunoglobulin G Donor-Specific Anti-HLA Antibody Subclasses and Kidney Allograft Antibody-Mediated Injury

HLA donor specific antibody (DSA) mediates injury by both complement dependent and independent mechanisms, including direct signaling via HLA antigens and via Fc receptor (FcR) engagement. Functional assessment of DSA has historically been limited to complement-based assays. FcR binding capacity and IgG isotypes provide complementary approaches for assessment of pathogenic potential of DSA. Assessment of the effects of proteasome inhibitor (PI) based treatment on global immunodominant DSA (iDSA) functional capacity has not been seen previously. We present analysis of comprehensive iDSA functional capacity assessment following PI-based treatment.

Methods: Serum samples obtained at the time of AMR diagnosis and following AMR treatment were analyzed by HLA single antigen bead (SAB) microarrays, C1q assay, IgG isotype-specific SAB assays (IgG1, IgG2, IgG3, IgG4), and FcR binding assays (performed per standard SOPs for the laboratory-developed test). iDSA FcR binding strength and incidence of IgG3 iDSA were compared pre- and post-treatment with a PI-based regimen.

Results: 51 patients with late AMR/MAR were analyzed (Table 1). Patients expressed reduced proportions of strong iDSA FcR binding capacity (75% vs 53%, p=0.003) and IgG3+ iDSA (73.5% vs. 41.2%, p=0.03) post-treatment. Other results listed in Figure 1.

Conclusions:

1)Treatment with a PI-based regimen reduced iDSA FcR binding strength and IgG3 binding strength in patients with late AMR/MAR

2)Patient with high serum creatinine at the time of rejection diagnosis experienced higher DCGS

3)Impact of comprehensive (FcR, C1q, and IgG isotype) functional analysis in the setting of late AMR/MAR warrants further evaluation

CITATION INFORMATION: Lichvar A, Portwood E, Brailey P, Tremblay S, Shields A, Abu Jawdeh B, Kremer J, Paterno F, Alloway R, Girnita A, Woodle E. Comprehensive Functional Assessment of the Pathogenic Potential of Immunodominant Donor Specific Antibody Following Treatment of Late Antibody Mediated Rejection or Mixed Acute Rejection. Am J Transplant. 2017;17 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Lichvar A, Portwood E, Brailey P, Tremblay S, Shields A, Jawdeh BAbu, Kremer J, Paterno F, Alloway R, Girnita A, Woodle E. Comprehensive Functional Assessment of the Pathogenic Potential of Immunodominant Donor Specific Antibody Following Treatment of Late Antibody Mediated Rejection or Mixed Acute Rejection. [abstract]. Am J Transplant. 2017; 17 (suppl 3). https://atcmeetingabstracts.com/abstract/comprehensive-functional-assessment-of-the-pathogenic-potential-of-immunodominant-donor-specific-antibody-following-treatment-of-late-antibody-mediated-rejection-or-mixed-acute-rejection/. Accessed January 23, 2021.

« Back to 2017 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
    • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
    • Penis Transplantation: First U.S. Experience.
    • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
    • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
    • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
    • Home
    • Search
    • Penis Transplantation: First U.S. Experience.
    • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
    • Low GFR after Kidney Donation Is Not Chronic Kidney Disease

    Visit Our Partner Sites

    American Transplant Congress (ATC)

    Visit the official site for the American Transplant Congress »

    American Journal of Transplantation

    The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

    American Society of Transplantation (AST)

    An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

    American Society of Transplant Surgeons (ASTS)

    The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

    Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

    Privacy Policy

    loading Cancel
    Post was not sent - check your email addresses!
    Email check failed, please try again
    Sorry, your blog cannot share posts by email.
    This site uses cookies: Find out more.