Purpose: To investigate the impact of BM-MDSCs on murine heart transplantation

Material and Methods:

Bone marrow cells were harvested and cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) for 4days to generate Gr1+ CD11b+ BM-MDSCs.

Vascularized heart from BALB/c mice was transplanted into the abdomen of C57BL/6 mice. Five million of BM-MDSCs was injected to transplanted mice immediately, 24 and 72 hours after transplantation. Graft survivals were compared to syngeneic and allogeneic control grafts.

Results: Ly6Glow-Ly6Chi monocytic MDSC were mainly generated and suppressed T cell activation via arginase I. Graft survival was significantly prolonged in BM-MDSCs group compared with allogeneic transplants (MST 37.2days vs 10days, p<0.05 t-test ). The number of infiltrated CD8+ and CD4+ Tcell in graft was also significantly lower in BM-MDSCs group. INF-γ and Granzyme B mRNA expression in graft were significantly decreased and foxp3 expression increased in BM-MDSCs group. Injected BM-MDSCs were not found neither spleen nor lymph node at 7 days after transplantation.

Conclusions: BM-MDSCs therapy is a potential option to protect graft rejection in organ transplantation.

CITATION INFORMATION: Uchida K., Fujimoto K., Takeda K., Bashuda H., Yin E., Okumura K. Bone Marrow Derived Myeloid Suppressor Cell (BM-MDSCs) Prolongs the Graft Survival in Murine Heart Transplantation Am J Transplant. 2017;17 (suppl 3).

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