Bone Marrow Derived Myeloid Suppressor Cell (BM-MDSCs) Prolongs the Graft Survival in Murine Heart Transplantation

K. Uchida, K. Fujimoto, K. Takeda, H. Bashuda, E. Yin, K. Okumura.

Atopy(Allergy) Research Center, Juntento University, Tokyo, Japan.

Meeting: 2018 American Transplant Congress

Abstract number: D276

Keywords: Mice, Rejection, T cell activation

Session Information

Session Name: Poster Session D: Late Breaking

Session Type: Poster Session

Date: Tuesday, June 5, 2018

Session Time: 6:00pm-7:00pm

Presentation Time: 6:00pm-7:00pm

Location: Hall 4EF

Purpose: To investigate the impact of BM-MDSCs on murine heart transplantation

Material and Methods:

Bone marrow cells were harvested and cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) for 4days to generate Gr1+ CD11b+ BM-MDSCs.

Vascularized heart from BALB/c mice was transplanted into the abdomen of C57BL/6 mice. Five million of BM-MDSCs was injected to transplanted mice immediately, 24 and 72 hours after transplantation. Graft survivals were compared to syngeneic and allogeneic control grafts.

Results: Ly6Glow-Ly6Chi monocytic MDSC were mainly generated and suppressed T cell activation via arginase I. Graft survival was significantly prolonged in BM-MDSCs group compared with allogeneic transplants (MST 37.2days vs 10days, p<0.05 t-test ). The number of infiltrated CD8+ and CD4+ Tcell in graft was also significantly lower in BM-MDSCs group. INF-γ and Granzyme B mRNA expression in graft were significantly decreased and foxp3 expression increased in BM-MDSCs group. Injected BM-MDSCs were not found neither spleen nor lymph node at 7 days after transplantation.

Conclusions: BM-MDSCs therapy is a potential option to protect graft rejection in organ transplantation.

CITATION INFORMATION: Uchida K., Fujimoto K., Takeda K., Bashuda H., Yin E., Okumura K. Bone Marrow Derived Myeloid Suppressor Cell (BM-MDSCs) Prolongs the Graft Survival in Murine Heart Transplantation Am J Transplant. 2017;17 (suppl 3).

To cite this abstract in AMA style:

Uchida K, Fujimoto K, Takeda K, Bashuda H, Yin E, Okumura K. Bone Marrow Derived Myeloid Suppressor Cell (BM-MDSCs) Prolongs the Graft Survival in Murine Heart Transplantation [abstract]. https://atcmeetingabstracts.com/abstract/bone-marrow-derived-myeloid-suppressor-cell-bm-mdscs-prolongs-the-graft-survival-in-murine-heart-transplantation/. Accessed May 9, 2025.

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