Background: Renal ischemia reperfusion injury (IRI) would highly increase the risk of delayed graft function (DGF) and rejection, and subsequently decrease the long-term graft survival rate in renal transplantation. However, effective treatments are still limited. Baicalin, a flavonoid glycoside isolated from Scutellaria baicalensis, has been widely applied in clinical treatment for various inflammatory diseases, based on its anti-inflammation and anti-apoptosis function. But its role in renal IRI remains unexplored.
Methods: Male wistar inbred rats were performed IRI experiments using 45 minutes of ischemia to the left kidney followed by nephrectomy
of the right kidney. Rats were euthanized at 24 hours after reperfusion. Baicalin at doses of 1 mg/kg, 10 mg/kg, 100 mg/kg was intraperitoneally injected 30 minutes before pedicle clamping. The activation of NF-kB pathway and caspase-3 mediated apoptosis were evaluated.
Results: Baicalin treatment at doses of 10 mg/kg and 100 mg/kg significantly decreased serum creatinine and blood urea nitrogen level when compared with IRI group. Pro-inflammatory cytokines, including IL-1, IL-6 and TNF-a, were also down regulated. HE staining, TUNEL detection, and MPO immunohistochemistry indicated milder pathological injury, less TEC apoptosis, and decreased leukocyte infiltration with baicalin treatment at doses of 10 mg/kg and 100 mg/kg as well. Activation status of NF-ΚB pathway was further investigated. Western blot analysis showed that cytoplasm p-IKK, p-I-ΚB, p-NF-ΚB, and nuclear NF-ΚB level were decreased in groups with 10 mg/kg and 100 mg/kg baicalin treatment. In addition, protein level of cleaved caspase-3, caspase-9 and bax were decreased in these two groups. At the same time, the protein level of bcl-2 was increased when compared with IRI group.
Conclusion: These data suggested that baicalin at doses of 10 mg/kg and 100 mg/kg mediates renal IRI protection through inhibition of NF-ΚB pathway and mitochondria mediated apoptosis.
To cite this abstract in AMA style:Lin M, Li L, Pokhrel G, Qi G, Wang J, Li L, Xu M, Rong R, Zhu T. Baicalin Mediates Renal IRI Protection through Inhibition of NF-κB Pathway and Mitochondria Mediated Apoptosis [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/baicalin-mediates-renal-iri-protection-through-inhibition-of-nf-b-pathway-and-mitochondria-mediated-apoptosis/. Accessed October 29, 2020.
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