Background: Apolipoprotein-L1 (APOL1) risk variants have emerged as a predictor of renal disease in individuals of African Ancestry (AA). The effect of APOL1 risk variants on the Living Kidney Donors (LD) renal function has been rarely reported. We investigated the effect of APOL1 genotype on LD kidney function post donation.

Methods: We reviewed prospectively collected data on 203 LD (83 male, mean age 43.5, 18-76 years). Genomic DNA was extracted from stored blood samples and three APOL1 single nucleotide polymorphisms (SNPs) were amplified using primers. The product was sequenced using the forward primer on the ABI 3130xl. The variants typed were (rs73885319 and rs60910145) are missense mutations in the last exon of the APOL1 gene (S342G and I384M) and (rs71785313), a six base-pair deletion leading to the deletion of two amino acids (delN388/Y389) in the last exon of the APOL1 gene. Sequences were evaluated using Mutation Surveyor software. 24h urine collection Creatinine Clearance (crcl) was utilised as a measure of renal function.

Results: 203 LD were included, 65 Asian (26 male, mean age 42.2, 18-76 years ), 55 AA (28 male, mean age 39.3, 19-64 years), and 83 Caucasian (29 male, mean age 47.4, 21-69 years ), with a mean follow up 52.1+32.4 months. Two APOL1 risk alleles were found in 26 (47.3%) AA, 2 (3.1%) Asian and none of the Caucasian LD. (p<0.001) In the AA cohort, the mean CrCl was similar in LD with >2 and 0-1 risk alleles pre transplantation (124.9+28.2 vs 111.7+26.4 ml/min, p=0.08) and at the end of follow up (98.4+31.2 vs 102.8+31.5 ml/min, p=0.6). The crcl decline per annum did not differ between AA with >2 and 0-1 risk alleles (-7.1+11.8 vs -4.24+15.1ml/min, p=0.45). Multivariate linear regression analysis showed that predictors for crcl at the end of follow up included age (Beta = -0.75, p < .01), and crcl pre transplant (Beta = 0.33, p =0.04), while APOL1 risk alleles (Beta = -9.5, p =0.24), and gender (Beta = -8.9, p =0.29) were not significant. The overall model fit was R^2 = 0.24

Conclusion: In this single center study, with medium term follow up, the presence of APOL1 risk alleles did not affect post donation renal function in donors of AA.

CITATION INFORMATION: Koutroutsos K, Sergeant R, Powell F, Freeman C, Charif R, Galliford J, Brookes P, Taube D, Loucaidou M. APOL1 Genotype Does Not Affect Donor Renal Function Post Live Kidney Donation. Am J Transplant. 2017;17 (suppl 3).

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