Articles tagged "Transcription factors"

2019 American Transplant Congress
RNA Splicing and Lineage Progression: Identification of an Integrated Splicing Pattern in Endodermal Lineage Progression
J. Fair¹, N. Liu¹, S. G. Widen², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX
*Purpose: Epigenetic states such as histone modifications and DNA methylation that influence transcription are well-characterized regulators of stem cell pluripotency and differentiation to specific lineages.…
2018 American Transplant Congress
Central Role of Mef2 Transcription Factors in Regulation of Foxp3 Expression and Treg Function
A. Samanta,¹ L. Wang,¹ R. Han,¹ W. Hancock.^1,2
¹CHOP, Philadelphia; ²UPenn, Philadelphia.
Foxp3+ Tregs express 4 class IIa Hdac enzymes (Hdac4, 5, 7 & 9) with little or no catalytic activity and yet when deleted, at least…
2018 American Transplant Congress
Ablation of IRF4 Induces Transplant Tolerance by Driving Intrinsic T Cell Dysfunction
J. Wu,^1,2 H. Zhang,¹ X. Shi,¹ X. Xiao,¹ Y. Fan,¹ J. Xia,² R. Sciammas,^1,3 X. Li,^1,3 W. Chen.^1,3
¹Center for Immunobiology & Transplant Science, Houston Methodist Research Institute, Houston, TX; ²Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; ³Department of Surgery, Weill Cornell Medical College of Cornell University, New York, NY.
How transplant rejection is regulated on a transcriptional level remains unclear. We found that T cell-specific conditional knockout (cKO) of the transcription factor IRF4 in…
2018 American Transplant Congress
A 3C Approach Drives Epigenetic Fixation of Foxp3 Expression and Stabilizes Treg Cell Lineage and Therapeutic Function
X. Xiao, X. Zhang, J. Li, S. Chen, P. Lan, Y. Dou, W. Chen, X. Li.
Immunobiology & Transplant Research Center, Houston Methodist Hospital, Houston, TX.
The instability of the regulatory T (Treg) cell lineage precludes their therapeutic potential in transplantation and autoimmune diseases. Herein, we invented a novel approach to…
2018 American Transplant Congress
Endothelial Progenitor Cell-Derived Extracellular Vesicles Inhibit Kidney Ischemia-Reperfusion Injury through the Transfer of Specific microRNA and mRNA Coding for the Complement Inhibitors CD55, CD59, Factor H and for the Transcription Factor NRF2: Relevance for Delayed Kidney Graft Function
V. Cantaluppi,¹ D. Medica,¹ S. Dellepiane,¹ G. Merlotti,¹ A. Airoldi,¹ M. Quaglia,¹ G. Castellano,² L. Gesualdo,² G. Camussi.³
¹Nephrology and Kidney Transplantation Unit, University of Piemonte Orientale (UPO), Novara, Italy; ²Nephrology, Dialysis and Kidney Transplantation Unit, University of Bari, Bari, Italy; ³Department of Medical Sciences, University of Torino, Torino, Italy.
Complement activation and oxidative cell damage are hallmarks of delayed kidney graft function (DGF) due to ischemia-reperfusion injury (IRI). Endothelial progenitor cells (EPC) protect from…
2018 American Transplant Congress
Galangin Promotes Treg Development and Function
F. Liang,¹ H. Fang,¹ R. Zhang,¹ Q. Chen,² J. Ochando,³ Y. Ding,¹ J. Xu.¹
¹Department of Immunology, Capital Medical University, Beijing, China; ²Department of Thoracic Surgery, Chaoyang Hospital, Beijing, China; ³Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
Introduction: FoxP3+ Treg cells are central in controlling immune responses and transplant tolerance. CD73 (ecto-5'-nucleotidase), which serves to convert AMP to adenosine, is essential for…
2018 American Transplant Congress
Rapamycin Therapy Impairs Treg Expression of CXCR3 and Limits Treg-Dependent Survival of Vascularized Composite Allotransplants
H. Xu,¹ Z. Chen,² W. Hancock,^3,4 S. Levin,⁵ Y. Zhang.¹
¹Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Medicine School of Shanghai Jiaotong University, Shanghai, China; ²Institute of Immunology, University of Pennsylvania, Philadelphia; ³Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia; ⁴Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia; ⁵Orthopaedic Surgery and Plastic Surgery, Hospital of the University of Pennsylvania, Philadelphia.
Vascularized Composite Allotransplantation (VCA) is a procedure to restore limb function or facial appearance. Rapamycin is used in efforts to preserve host Treg function and…
2018 American Transplant Congress
Krüppel-Like Factor 15 is a Key Suppressor of the Glomerular Epithelial Cell Fibrosis under Centrifugal Force Driven Pressure
M. Yu,¹ Y. Kim,¹ H. Lee,¹ J. Lee,² R. Cha,³ C. Lee,⁴ Y. Kim,¹ S. Yang.⁵
¹Seoul National University Hospital, Seoul, Korea; ²Seoul National University Boramae Medical Center, Seoul, Korea; ³National Medical Center, Seoul, Korea; ⁴Cheju Halla General Hospital, Jeju, Korea; ⁵Seoul National University Kidney Research Institue, Seoul, Korea.
Hypertension is known to be responsible for a reduction in the number of functional nephrons of glomeruli due to fibrosis, which develops into a chronic…
2018 American Transplant Congress
Shikonin Inhibits Th17 Development through Repressing STAT3 Activation
H. Fang,¹ R. Zhang,¹ F. Liang,¹ Q. Chen,² J. Ochando,³ Y. Ding,¹ J. Xu.¹
¹Department of Immunology, Capital Medical University, Beijing, China; ²Department of Thoracic Surgery, Chaoyang Hospital, Beijing, China; ³Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
Background: Th17-mediated inflammatory responses are detrimental for allograft acceptance and long-term survival after transplantation. Shikonin is a naphthoquinone pigment isolated from a traditional medicinal herb…
2018 American Transplant Congress
Urine Podocin mRNA to Nephrin mRNA Ratio as a Marker of "Podocyte Stress" among Kidney Allografts Losing Function
A. Naik,¹ J. Aqeel,¹ D. Cibrik,² M. Samaniego,¹ S. Wang,¹ M. Chowdhury,¹ F. Afshinnia,¹ R. Wiggins.¹
¹Univ of Michigan, Ann Arbor; ²Univ of Kansas, Kansas City.
Increasing data supports the hypothesis that long term allograft loss-of-function is associated with progressive glomerulosclerosis (GS). In models of progressive GS the ratio of two…