2019 American Transplant Congress
Absolute Quantification of Micro RNA and Messenger RNA Using a Customized Amplicon in Real Time Quantitative Polymerase Chain Reaction Assays
*Purpose: Transcript abundance in biological specimens quantified by RT-PCR assay is typically expressed as fold change between groups. Absolute quantification is preferable to relative quantification…2019 American Transplant Congress
Quantitative Epstein-Barr Virus Viral Load Monitoring in Pediatric Kidney Transplantation
*Purpose: Epstein Barr virus (EBV) belongs to the family of herpesvirus and is associated with a wide range of malignancies, including post-transplant lymphoproliferative disorder (PTLD).…2018 American Transplant Congress
Identification of Noninvasive and Mechanistically-Informative Biomarkers Predictive of Reversal of Acute Cellular Rejection (ACR) in Kidney Allografts
1University of Michigan, Ann Arbor, MI; 2Weill Cornell Medical College, New York City, NY.
Objective: We determined whether urinary cell mRNAs predict response to anti-rejection therapy in kidney allograft recipients with Banff grade IA or higher ACR biopsies.Methods: We…2018 American Transplant Congress
Intragraft MicroRNA Transcriptome of Acute Antibody Mediated Rejection of Human Kidney Allografts
1Weill Cornell Medicine, New York; 2Rockefeller University, New York.
BackgroundMicroRNAs (miRNA) are master regulators of gene expression and are potential therapeutic targets. Intragraft miRNA transcriptome of acute antibody rejection (AMR) of human kidney allograft…2018 American Transplant Congress
Y-Adapter Mediated Next Gen Sequencing of BKPyV from Archived FFPE Tissue
1Pathology, Michigan Medicine, Ann Arbor; 2Michigan Medicine, Ann Arbor.
Background: In a subset of renal transplant recipients with urothelial carcinoma, tumor cells show universal expression of BK Polyomavirus (BKPyV) proteins, a hallmark of oncoviruses.…2017 American Transplant Congress
NFAT-Regulated Cytokine Gene Expression for Immune Monitoring in Kidney Transplant Recipients – A Randomized Controlled Trial.
Purpose: To demonstrate that a pharmacodynamics assay using Real Time (RT)-PCR to measure residual expression (RE) of nuclear factor of activated T cell (NFAT)-dependent cytokines…2017 American Transplant Congress
Impact of Peri-Transplant Viral Upper Respiratory Infection on Pediatric Liver Transplant Outcomes.
BACKGROUND: Viral upper respiratory infection (vURI) is common in children and its effect at the time of liver transplant (LT) on the post-operative outcomes is…2017 American Transplant Congress
Calibration of BK Virus Nucleic Acid Amplification Testing to the 1st WHO International Standard for BK Virus.
Significant inter-assay variability in the quantification of BK virus (BKV) DNA has limited the broad applicability of thresholds for the management of BKV infection in…2017 American Transplant Congress
Cost Effectiveness of Post-Prophylaxis Screening Strategies for Cytomegalovirus Viremia in CMV D+R- Kidney Transplant Recipients.
Purpose: To assess cost-effectiveness(CE) of screening for CMV after 6 months of valganciclovir prophylaxis in CMV high-risk (D+R-) kidney transplant recipients.Methods: A Markov model was…2017 American Transplant Congress
Cytomegalovirus Viral Load: Characterization of Results from Clinical Specimens.
Viracor Eurofins, Lees Summit, MO
Cytomegalovirus (CMV) infection, a frequent complication affecting solid organ and hematopoietic stem cell transplant recipients, is commonly diagnosed and monitored by quantitative PCR (qPCR) methods.…
American Transplant Congress abstracts