Articles tagged "Perfusion solutions"

2020 American Transplant Congress
Using an Ex Vivo Blood-Free Model of Preservation and Reperfusion to Evaluate the Effects of Subnormothermic Perfusion with Hydrogen Sulfide on DCD Porcine Renal Grafts
S. Juriasingani¹, A. Ruthirakanthan¹, M. Richard-Mohamed², M. Zhang¹, M. Levine¹, E. Sogutdelen¹, M. Mandurah¹, A. Sener¹
¹University of Western Ontario, London, ON, Canada, ²London Health Sciences Centre, London, ON, Canada
*Purpose: The need for donor blood complicates the clinical translation of normothermic and subnormothermic perfusion for preservation and graft function evaluation. As such, hemoglobin-based oxygen…
2020 American Transplant Congress
A Technique for Ex-Vivo Perfusion of Donor Kidneys for Transplantation in the Mouse
R. Plenter¹, S. Jain², J. J. Pomposelli¹, T. Nydam¹, A. Jani²
¹Pulmonary Division, University of Colorado, Aurora, CO, ²Renal Division, University of Colorado, Aurora, CO
*Purpose: Ex-vivo perfusion of donor kidneys is widely employed in the US and significantly reduces DGF. Development of a pre-clinical perfusion technique may provide an…
2019 American Transplant Congress
Graft Perfusion with Alvespimycin, an HSP90 Inhibitor, Suppresses IL-2 and IL-12p40 Production in Graft-Infiltrating Cells, Leading to Prolongation of Graft Survival in Murine Heart Transplantation
T. Tanaka, T. Maehana, N. Masumori
Department of Urology, Sapporo Medical University, Sapporo, Japan
*Purpose: We have previously demonstrated that administration of a heat shock protein 90 (HSP90) inhibitor prolonged the allograft survival in murine skin and heart transplantations.…
2019 American Transplant Congress
Defatting Steatotic Rat Livers during Ex Situ Normothermic Perfusion Improves Lactate Clearance and Bile Quality
S. Raigani¹, C. Carroll², S. Cronin², C. Pendexter², I. Rosales³, M. Yarmush¹, K. Uygun¹, H. Yeh¹
¹Transplant Surgery, Massachusetts General Hospital, Boston, MA, ²Center for Engineering in Medicine, Shriners Childrens Hospital, Boston, MA, ³Pathology, Massachusetts General Hospital, Boston, MA
*Purpose: Donor livers with moderate-to-severe steatosis are not generally used for transplantation because of poor post-transplant function. Ex situ normothermic machine perfusion (ENMP) provides an…
2019 American Transplant Congress
Achieving Robust Targeting of Antibody-Conjugated Nanoparticles Delivered during Ex Vivo Machine Perfusion of Human Kidneys
J. R. DiRito¹, S. Hosgood¹, M. L. Nicholson¹, G. Tietjen²
¹Surgery, University of Cambridge, Cambridge, United Kingdom, ²Surgery, Yale School of Medicine, New Haven, CT
*Purpose: In this study we seek to improve targeted nanoparticle (NP) delivery during ex-vivo normothermic machine perfusion through fibrinolysis for more homogenous treatment of human…
2019 American Transplant Congress
Flow Cytometric Analysis of Leukocyte Migration during Ex Vivo Lung Perfusion with CXCR4 Inhibition
J. Yerxa¹, Q. Gao¹, R. P. Davis², M. Zhang¹, U. Scheuermann¹, W. Parker¹, M. G. Hartwig², A. Barbas¹
¹General Surgery, Duke University, Durham, NC, ²Thoracic Surgery, Duke University, Durham, NC
*Purpose: Ex vivo lung perfusion (EVLP) has emerged as a preservation technique utilized to evaluate candidate donor lungs in a metabolically active state. This also…
2019 American Transplant Congress
Normothermic Ex Situ Liver Perfusion with Different Clinical Perfusates and Their Effect on Pig Liver Transplant Outcomes in a Donation after Circulatory Death Model
I. Linares-Cervantes, D. Kollmann, T. Goto, J. Echeverri, M. Kaths, M. Hamar, P. Urbanellis, L. Mazilescu, R. Rosales, C. Bruguera, F. Oquendo, S. Ganesh, O. Adeyi, P. Yip, N. Selzner, M. Selzner
Toronto General Hospital, Toronto, ON, Canada
*Purpose: Human-albumin/Dextran (HA-D), Bovine-gelatin (BG) and packed red blood cells plus plasma have been used in European and North-American clinical trials of normothermic ex-situ liver…
2019 American Transplant Congress
High Urinary Neutrophil Gelatinase Lipocalins (NGAL) Levels in Kidney Perfusates as Predictor of a Better Renal Function
A. Angeletti, C. Donadei, V. De Pace, I. Capelli, V. Corradetti, C. Zannini, F. Alviano, L. Bonsi, G. Comai, M. Ravaioli, G. La Manna
DIMES, S.Orsola-Malpighi Hospital, Bologna University, Bologna, Italy
*Purpose: Neutrophil gelatinase-associated lipocalin (NGAL) has recently emerged as a biomarker of different conditions, but the biological role remains unclear. We hypnotized that NGAL has…
2019 American Transplant Congress
Urine Recirculation Maintains Hemodynamics and Enhances Function in Normothermic Kidney Perfusion
A. Weissenbacher¹, C. D. Ceresa², D. Voyce³, J. Hunter², R. Ploeg², C. C. Coussios⁴, P. Friend²
¹Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria, ²Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom, ³OrganOx Limited, Oxford, United Kingdom, ⁴Oxford Institute of Biomedical Engineering, University of Oxford, Oxford, United Kingdom
*Purpose: The purpose was to compare urine recirculation (URC) and urine replacement with Ringer’s lactate in a porcine normothermic kidney perfusion (NKP) model using a…
2019 American Transplant Congress
Partial Freezing: Subzero Whole Liver Preservation In The Presence Of Ice
S. E. Cronin¹, C. A. Pendexter¹, R. J. deVries¹, S. Kimura², S. Ozer¹, P. D. Banik¹, S. Nagpal¹, H. Yeh², S. N. Tessier¹, K. Uygun¹, M. Toner¹
¹Center for Engineering in Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, ²Transplant Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA
*Purpose: We aim to develop a protocol to bank organs for several days, enabling global organ matching and potentially the utilization of organs unusable with…