Articles tagged "Mice"

2015 American Transplant Congress
Ischemia-Induced Endoplasmic Reticulum Stress Regulates Autophagy to Determine the Severity of Liver Ischemia Reperfusion Injury
H. Zhou,^1,2 J. Zhu,¹ S. Yue,¹ R. Busuttil,¹ J. Kupiec-Weglinski,¹ Y. Zhai.¹
¹The Dumont-UCLA Transplant Center, UCLA, Los Angeles, CA; ²Liver Transplant Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Both endoplasmic reticulum (ER) stress and autophagy have been implicated in the pathogenesis of ischemia and reperfusion injury (IRI). As essential cellular stress responses against…
2015 American Transplant Congress
Interleukin-17 Deficiency Attenuates Renal Allograft Injury and Prolongs Survival in a Murine Transplantation Model
T. Kwan,^1,2 S. Chadban,^1,2 J. Ma,^1,2 S. Bao,^2,3 S. Alexander,^2,4 H. Wu.^1,2
¹Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia; ²Renal Medicine, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia; ³Discipline of Pathology, School of Medical Sciences, Sydney, New South Wales, Australia; ⁴Centre for Kidney Research, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.
Aim:To investigate the role of IL-17 in allograft rejection using a murine model of fully MHC-mismatched renal transplantation.Methods:Life-sustaining kidney transplants were performed in nephrectomized mice:…
2015 American Transplant Congress
Leukocyte Associated Immunoglobulin-Like Receptor 1 inhibits Th1 But Enhances Th17 Responses
V. Agashe,¹ M. Keller,² L. Haynes,¹ E. Jankowska-Gan,¹ J. Sullivan,¹ J. Kernien,¹ H. Suzuki,³ D. Wilkes,⁴ W. Burlingham.¹
¹Surgery, UW Madison, Madison, WI; ²Tulane University, New Orleans, LA; ³Chiba Graduate School of Medicine, Chiba, Japan; ⁴Indiana University School of Medicine, Indianapolis, IN.
Background: Leukocyte Associated Immunoglobulin-like Receptor 1 (LAIR1), is a transmembrane receptor expressed by immune cells. It recognizes collagen repeats via its extracellular domain and has…
2015 American Transplant Congress
Emerging Application of Active Immune Targeted Delivery in Transplantation
M. Uehara,¹ A. Jamil,¹ M. McGrath,¹ Z. Solhjou,¹ N. Banouni,¹ S. Tullius,² C. Jianjun,³ R. Abdi.¹
¹Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical Shcool, Boston, MA; ²Division of Transplant Surgery, Brigham and Women's Hospital, Boston, MA; ³Materials Science and Engineering, University of Illinois at Urbana-Champaign, Champaingn, IL.
Targeted nanodelivery has created enormous excitement as a transformative approach to deliver therapeutics to tissues of interest. In recent years, we have made significant progress…
2015 American Transplant Congress
Proteomic Study Identified a Distinct Panel of Protein Expression in the ATP-Competitive MTOR Kinase Inhibitor Conditioned Dendritic Cells Compared to Rapamycin
T. Ma,¹ Y. Pei,¹ Y. Han,² R. Tao.¹
¹Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China; ²Department of Pathology, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China.
Purpose: Dendritic cells (DCs) are key players in both innate and adaptive immunity, as well as in immune tolerance. The mammalian target of rapamycin (mTOR)…
2015 American Transplant Congress
Macrophage-Specific SIRT1 Regulates HO-1 Mediated Anti-Inflammatory and Cytoprotective Pathways
K. Nakamura,¹ M. Zhang,² B. Ke,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California, Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California, Los Angeles, Los Angeles, CA.
Background/Aim: Sirtuin1 (SIRT1), a NAD+-dependent type III histone/protein deacetylase, is essential for resistance against liver ischemia-reperfusion injury (IRI). We asked whether SIRT1 may affect cytoprotective…
2015 American Transplant Congress
The Gut Microbiota Regulates Murine Cardiac Transplant Outcome
C. Brinkman,¹ L. Hittle,¹ E. Mongodin,¹ W. Fricke,² J. Bromberg.¹
¹University of Maryland, Baltimore, MD; ²University of Hohenheim, Stuttgart, Germany.
Hypothesis: The gut microbiota regulates systemic immunity. Different characteristics of the microbiota can be defined that have pro- or anti-inflammatory effects. We tested the hypothesis…
2015 American Transplant Congress
DNA Methytransferase 1 Inhibition Potently Suppresses T Cell Cycle Progression Through Demethylation of the Key Cell Cycle Inhibitors p15, p16, p21 and p27
T. Ma, Y. Pei, R. Tao.
Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China.
Background: Epigenetic mechanisms like acetylation and methylation have been gradually appreciated to play a critical role in the generation and function of various immune cells.…
2015 American Transplant Congress
The Role of an Intragraft Vascularized Bone Marrow Niche in Establishing Mixed Chimerism and Allograft Acceptance
M. Fryer, B. Oh, G. Furtmüller, G. Raimondi, G. Brandacher.
Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD.
Background: Vascularized composite allotransplantation has emerged as a viable treatment option for patients with devastating tissue loss not amenable to traditional reconstructive techniques. Different from…
2015 American Transplant Congress
Oxidative Stress During Organ Recovery Influences Transplant Vasculopathy Development
M. Maglione,¹ G. Riede,¹ B. Cardini,¹ D. Bernhard,² B. Messner,² K. Watschinger,³ C. Steger,⁴ G. Brandacher,⁵ J. Pratschke,¹ E. Werner,³ R. Oberhuber.¹
¹Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria; ²Cardiac Surgery Research Laboratory, Department of Surgery, Vienna Medical University, Vienna, Austria; ³Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Innsbruck, Austria; ⁴Institute of Pathology, Innsbruck Medical University, Innsbruck, Austria; ⁵Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Transplant vasculopathy is still a major obstacle to long-term graft survival. Pharmacological strategies aiming at preventing it are still lacking. Herein we show that the…