Articles tagged "Mice"

2015 American Transplant Congress
Proteomic Study Identified a Distinct Panel of Protein Expression in the ATP-Competitive MTOR Kinase Inhibitor Conditioned Dendritic Cells Compared to Rapamycin
T. Ma,¹ Y. Pei,¹ Y. Han,² R. Tao.¹
¹Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China; ²Department of Pathology, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China.
Purpose: Dendritic cells (DCs) are key players in both innate and adaptive immunity, as well as in immune tolerance. The mammalian target of rapamycin (mTOR)…
2015 American Transplant Congress
Macrophage-Specific SIRT1 Regulates HO-1 Mediated Anti-Inflammatory and Cytoprotective Pathways
K. Nakamura,¹ M. Zhang,² B. Ke,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California, Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California, Los Angeles, Los Angeles, CA.
Background/Aim: Sirtuin1 (SIRT1), a NAD+-dependent type III histone/protein deacetylase, is essential for resistance against liver ischemia-reperfusion injury (IRI). We asked whether SIRT1 may affect cytoprotective…
2015 American Transplant Congress
The Gut Microbiota Regulates Murine Cardiac Transplant Outcome
C. Brinkman,¹ L. Hittle,¹ E. Mongodin,¹ W. Fricke,² J. Bromberg.¹
¹University of Maryland, Baltimore, MD; ²University of Hohenheim, Stuttgart, Germany.
Hypothesis: The gut microbiota regulates systemic immunity. Different characteristics of the microbiota can be defined that have pro- or anti-inflammatory effects. We tested the hypothesis…
2015 American Transplant Congress
DNA Methytransferase 1 Inhibition Potently Suppresses T Cell Cycle Progression Through Demethylation of the Key Cell Cycle Inhibitors p15, p16, p21 and p27
T. Ma, Y. Pei, R. Tao.
Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China.
Background: Epigenetic mechanisms like acetylation and methylation have been gradually appreciated to play a critical role in the generation and function of various immune cells.…
2015 American Transplant Congress
The Role of an Intragraft Vascularized Bone Marrow Niche in Establishing Mixed Chimerism and Allograft Acceptance
M. Fryer, B. Oh, G. Furtmüller, G. Raimondi, G. Brandacher.
Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD.
Background: Vascularized composite allotransplantation has emerged as a viable treatment option for patients with devastating tissue loss not amenable to traditional reconstructive techniques. Different from…
2015 American Transplant Congress
Oxidative Stress During Organ Recovery Influences Transplant Vasculopathy Development
M. Maglione,¹ G. Riede,¹ B. Cardini,¹ D. Bernhard,² B. Messner,² K. Watschinger,³ C. Steger,⁴ G. Brandacher,⁵ J. Pratschke,¹ E. Werner,³ R. Oberhuber.¹
¹Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innsbruck, Austria; ²Cardiac Surgery Research Laboratory, Department of Surgery, Vienna Medical University, Vienna, Austria; ³Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Innsbruck, Austria; ⁴Institute of Pathology, Innsbruck Medical University, Innsbruck, Austria; ⁵Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Transplant vasculopathy is still a major obstacle to long-term graft survival. Pharmacological strategies aiming at preventing it are still lacking. Herein we show that the…
2015 American Transplant Congress
Allograft Class II MHC and CD40 Expression Are Required for Endogenous Memory CD8 T Cell Proliferation in Cardiac Allografts Subjected to Prolonged Cold Ischemic Storage
T. Tanaka,^1,2 H. Tsuda,^1,3 N. Kohei,^1,4 D. Kish,¹ W. Baldwin,¹ A. Valujskikh,¹ R. Fairchild.¹
¹Immunology, Cleveland Clinic, Cleveland, OH; ²Urology, Sapporo Medical University, Sapporo, Japan; ³Urology, Osaka University, Osaka, Japan; ⁴Urology, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan.
Endogenous memory CD8 T cells infiltrate MHC-mismatched cardiac allografts and produce IFN-γ in response to donor class І MHC within 24 hrs after graft reperfusion…
2015 American Transplant Congress
Single Infusion of Donor-Type Splenocytes Treated With Extracorporeal Photopheresis Prior to Transplantation Significantly Prolongs Cardiac Allograft Survival in Mice
J. Schneiderman,¹ J. Ye,² X. Yeap,² M. Caralt,² J.-J. Wang,² Z. Zhang.²
¹Ped Hematology/Oncology/Stem Cell Transplant, Feinberg School of Medicine Northwestern University, Chicago, IL; ²Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL.
Background: Extracorporeal Photopheresis (ECP) is an immune therapy in which blood leukocytes are treated with 8-methoxypsoralen (8-MOP), exposed to UV-A light, and re-infused. This study…
2015 American Transplant Congress
Defining and Directing T Cell Subsets Involved in Rejection of Vascularized Composite Allografts
M. Fryer, G. Furtmùller, B. Oh, S. Reddy, K. Sarhane, G. Raimondi, G. Brandacher, D. Cooney.
Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD.
Purpose: Wider utilization of VCA is hampered by the need for long-term immunosuppression. Barriers to more targeted immunosuppression in VCA include (1) limited knowledge of…
2015 American Transplant Congress
A Mouse Model of Pneumonitis Induced by Candida Albicans Infection After Allogeneic Skin Transplantation
A. Yang, Z. Guo, W. Ju, Y. Ma, A. Hu, Y. Chen, M. Chen, C. Wu, X. He.
Organ Transplantation Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Aims:Our study was to build up a mouse model of pneumonitis induced by candida albicans infection after allogeneic skin transplantation.Methods:We used 10 male C57BL/6J mice…