Articles tagged "Mice, knockout"

2019 American Transplant Congress
Graft TIM4 Deficiency Protects Liver from Ischemia Reperfusion Injury: From Mouse to Human
S. Kageyama¹, K. Kadono¹, H. Hirao¹, K. Nakamura¹, T. Ito², K. J. Dery¹, D. Oncel¹, B. Ke¹, F. M. Kaldas², R. W. Busuttil², J. W. Kupiec-Weglinski¹
¹Surgery, Dumont-UCLA Transplant Center, Los Angeles, CA, ²Surgery, Ronald Reagan UCLA Medical Center, Los Angeles, CA
*Purpose: Liver ischemia-reperfusion injury (IRI) is a major risk factor for early graft dysfunction and rejection in orthotopic liver transplantation (OLT). We and others have…
2019 American Transplant Congress
Withaferin A as a Possible Therapy for Chronic Allograft Nephropathy
T. Divanyan¹, D. Patel¹, E. Acosta¹, D. Howard², F. Jourd'heuil², D. Jourd'heuil², R. Lopez-Soler³
¹Albany Medical College, Albany, NY, ²Molecular and Cellular Physiology, Albany Medical College, Albany, NY, ³Surgery, Section of Transplantation, Albany Medical College, Albany, NY
*Purpose: One of the possible steps in the development of post-transplant interstitial fibrosis is epithelial to mesenchymal transition (EMT) of proximal renal tubular cells with…
2019 American Transplant Congress
CCR5-Mediated Recruitment of NK Cells to the Kidney is a Critical Step for Host Defense to Candida albicans Infection
H. R. Cho¹, V. G. Tran², N. N. Nguyen³, S. Lee⁴, S. W. Kang⁴, J. Kim⁴, H. J. Kim⁴, S. J. Park¹, J. S. Lee⁵, B. Kwon⁶
¹Department of Surgery, Ulsan University Hospital, Ulsan, Korea, Republic of, ²School of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea, Ulsan, Korea, Republic of, ³1School of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea, Ulsan, Korea, Republic of, ⁴Biomedical Research Center, Ulsan University Hospital, Ulsan, Korea, Republic of, ⁵Division of Nephrology, Department of Internal Medicine, Ulsan University Hospital, Ulsan, Korea, Republic of, ⁶Biomedical Research Center, Ulsan University Hospital, School of Medicine, University of Ulsan, Ulsan, Korea, Republic of
*Purpose: Candida albicans infection causes a high rate of mortality in immunocompromised or transplant patients. In this study, we investigated the role of CCR5 that…
2019 American Transplant Congress
Deficiency of IRE1a Ameliorates Renal Allograft Injury via Remodeling of Graft-Infiltrating Macrophages
L. Qiu¹, J. Wang¹, X. Yeap¹, S. Han¹, F. Zheng¹, D. Fang², Z. J. Zhang¹
¹Comprehensive Transplant Center, Department of Surgery, Northwestern University, Chicago, IL, ²Department of Pathology, Northwestern University, Chicago, IL
*Purpose: Inflammatory macrophages contribute to the development of ischemia-reperfusion injury (IRI) and allograft rejection. However, the underlying mechanisms by which macrophage mediate allograft injury and…
2019 American Transplant Congress
Lymphotoxin Beta Receptor Determines Lymph Node Structure for Treg Migration and Suppression of Graft Rejection
V. Saxena, W. Piao, L. Li, C. M. Paluskievicz, Y. Xiong, T. Simon, J. S. Bromberg
U Maryland, Baltimore, MD
*Purpose: Lymphotoxin beta receptor (LTβR) mediated signaling in lymphatic endothelium cells (LEC) is important for migration of regulatory T cells (Treg) to afferent lymphatic vessels…
2019 American Transplant Congress
Treg Lymphotoxin and Lymphatic Endothelial Cell Lymphotoxin Receptor Interactions Are Required for Treg Stability and Allograft Protection
V. Saxena, W. Piao, L. Li, C. M. Palusckievicz, Y. Xiong, T. Simon, J. S. Bromberg
U Maryland, Baltimore, MD
*Purpose: Regulatory T cells (Treg) restrain allograft rejection through suppressor functions that are dependent upon expression of the transcription factor Foxp3, their proper migration, and…
2019 American Transplant Congress
S1PR1 and S1PR4 Regulate T Cell Migration into Afferent Lymphatics
Y. Xiong¹, W. Piao¹, C. Brinkman¹, L. Li¹, J. Kulinski², A. Olivera², A. Cartier³, T. Hla⁴, K. Hippen⁵, B. Blazar⁶, S. Schwab⁷, J. Bromberg¹
¹Surgery, UMB, Baltimore, MD, ²NIH, Bethesda, MD, ³Boston Children's hospital, Boston, MA, ⁴Boston Children's, Boston, MA, ⁵U.Minnesota, Minneapolis, MN, ⁶U.Minnesota, Minneapolis, MD, ⁷NYU, NY, NY
*Purpose: T cells utilize S1PR1 to traffic from thymus into blood, and lymph nodes (LN) into efferent lymphatics. Whether T cell S1PR1 and S1PR4 regulate…
2019 American Transplant Congress
Depleting Laminin α5 in Lymph Node Stromal Cells Promotes Transplant Tolerance
L. Li, M. Shirkey, W. Piao, Y. Xiong, V. Saxena, T. Zhang, N. Toney, C. Paluskievicz, J. S. Bromberg
Surgery, U. Maryland, Baltimore, Baltimore, MD
*Purpose: Expression of stromal laminin α5β1γ1 (511) in lymph nodes (LN) is associated with immunity, while laminin α4β1γ1 (411) is associated with tolerance. The laminins…
2019 American Transplant Congress
Decay Accelerating Factor (DAF) Regulates Germinal Center B-cell and Alloantibody Formation
A. Cumpelik¹, G. Varano², D. Heja¹, D. Homann³, S. Lira⁴, D. Dominguez-Sola², P. S. Heeger¹
¹Nephrology, Mount Sinai School of Medicine, New York, NY, ²Tisch Cancer Institute, Mount Sinai School of Medicine, New York, NY, ³Medicine, Endocrinology, Diabetes and Bone Disease, Mount Sinai School of Medicine, New York, NY, ⁴Clinical Immunology, Mount Sinai School of Medicine, New York, NY
*Purpose: High affinity IgG antibodies including alloantibodies result from germinal center (GC) reactions in which somatic hypermutation and positive selection cause affinity maturation. When we…
2019 American Transplant Congress
B Cell-Specific IRE1a Deficiency Protects Renal Allografts from Rejection via Inhibition of Donor-Specific Antibodies and Induction of B Regulatory Cells
L. Qiu¹, J. Wang¹, X. Yeap¹, D. Fang², Z. J. Zhang¹
¹Comprehensive Transplant Center, Department of Surgery, Northwestern University, Chicago, IL, ²Department of Pathology, Northwestern University, Chicago, IL
*Purpose: Despite the progress in immunosuppressive regimens, antibody-mediated rejection remains a major contributor to renal allograft loss in recipients with donor-specific antibodies (DSA). IRE1a, an…