2022 American Transplant Congress
Targeted Delivery of Costimulation Inhibitor to Lymph Nodes Promotes Transplant Tolerance via Fibroblastic Reticular Cells
1Brigham and Women's Hospital, Boston, MA, 2University of Maryland School of Medicine, Baltimore, MD
*Purpose: The lymph node (LN) is the primary site of alloimmunity activation and regulation during transplantation. Fibroblastic reticular cells (FRCs), the prominent cells of the…2022 American Transplant Congress
Fibroblastic Reticular Cell Subset Transcriptional Reprogramming Differs in Response to Immunity versus Tolerance
1UMB, Baltimore, MD, 2Harvard University, Boston, MD
*Purpose: Lymph node (LN) fibroblastic reticular cells (FRCs) are central contributors to the LN niche. We hypothesized that FRC subsets reactive to immunity versus tolerance…2022 American Transplant Congress
Deletion of Fibroblastic Reticular Cell Derived Laminin A4 Impairs Lymph Node Vasculature and Conduits
1UMB, Baltimore, MD, 2Harvard University, Boston, MA
*Purpose: Fibroblastic reticular cells (FRCs) support lymph node (LN) structure by producing various functional extracellular matrix (ECM) components. We hypothesized that FRCs and their ECM…2022 American Transplant Congress
Immunosuppressants Alter Lymph Node Architecture and Create Distinct Microbiome Footprints
1University of Maryland School of Medicine, Baltimore, MD, 2University of Maryland, Baltimore, MD
*Purpose: There are complex interactions among immunosuppressants, antibiotics, and the gut microbiome on the host immune system. Microbiota can alter lymph node (LN) structure and…2021 American Transplant Congress
Laminins Differentially Regulate Tolerogenic T Cell Migration and Homing to Lymph Nodes
1Surgery, UMB, Baltimore, MD, 2CVID, UMB, Baltimore, MD, 3Harvard University, Boston, MA
*Purpose: Laminin α5 (Lama5) and α4 (Lama4), which are produced by lymph node (LN) stromal cells, influence CD4 T cell migration and stimulation. We hypothesized…2020 American Transplant Congress
Ex Vivo Generated Regulatory B Cells Can Suppress Donor-Specific CD4 T Cells Proliferation In Vivo
*Purpose: B cells expanded in vitro via TLR9 agonist CpG and activated by LPS, PMA and ionomycin have been previously denoted “TLR-Bregs” by our team.…2020 American Transplant Congress
Laminin Alpha5 Regulates T Cell Entry and Distribution in Lymph Nodes in Immunity
1UMB, Baltimore, MD, 2Harvard University, Boston, MA
*Purpose: Laminin α5 (Lama5) and α4 (Lama4) are lymph node (LN) stromal fibers whose expression ratio influences CD4+ T cell migration and stimulation. We hypothesized…2020 American Transplant Congress
Dissecting Humoral Alloimmune Sensitization: A Dynamic Interplay of Different Cellular Compartments
*Purpose: Humoral alloimmune memory is the main barrier for successful transplantation and is generated by a complex and compartmentalized B-cell immune response. Understanding the role…2020 American Transplant Congress
Costimulation Blockade-Based Maintenance Immunosuppression in a Sensitized Nonhuman Primate Kidney Allotransplantation Model
Department of Surgery, Duke University Medical Center, Durham, NC
*Purpose: Sensitized transplant recipients represent a challenging population in part due to the presence of donor-specific antibodies (DSA), which increase the risk of antibody-mediated rejection.…2020 American Transplant Congress
Characterization of Laminin Alpha 4 Conditional Knockout Mice: Model for Immune Stimulation
*Purpose: Lymph node (LN) stromal laminin α4 (Lama4) and α5 (Lama5) are associated with tolerance and immunity, respectively. We created a Lama4 conditional knockout (KO)…
American Transplant Congress abstracts