Articles tagged "Bone marrow"

2020 American Transplant Congress
BAFF Inhibition to Prevent Antibody Rebound Following Proteasome Inhibitor-Based Desensitization: A Randomized, Controlled Trial
S. Tremblay¹, A. Rike-Shields², A. Govil¹, M. Cardi³, P. Brailey¹, R. Alloway¹, E. Woodle¹
¹U Cincinnati, Cincinnati, OH, ²Surgery, U Cincinnati, Christ Hospital, Cincinnati, OH, ³Christ Hospital, Cincinnati, OH
*Purpose: Proteasome inhibitor (PI) therapy reduces bone marrow plasma cells and HLA antibodies (Abs) in renal transplant candidates. However, rebound is common in many patients…
2020 American Transplant Congress
IL-6 Receptor Blockade But Not IL-2 Treatment Contributes to Long-Term NHP Cardiac Allograft Survival in Transient Mixed Chimeras
K. Ahrens¹, J. M. O¹, W. Sommer², J. Morrissette¹, D. Becerra¹, P. M. Patel¹, T. Costa¹, A. Dehnadi¹, I. M. Hanekamp¹, J. S. Allan³, G. Benichou¹, J. C. Madsen⁴
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁴Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Long-term tolerance of cardiac allografts has been achieved in non-human primates (NHPs) using a mixed chimerism protocol and by co-transplanting a kidney from the…
2019 American Transplant Congress
Adipose Stem Cells Promote Composite Tissue Engraftment
N. Shockcor¹, B. Buckingham¹, W. Hassanein¹, D. Parsell¹, U. Dhru¹, B. Akinsiku², E. Elster², T. Davis², J. Gimble³, A. Nam¹, S. Bartlett¹, R. Barth¹
¹Surgery, University of Maryland, Baltimore, MD, ²Uniformed Services University of the Health Sciences, Bethesda, MD, ³LaCell LLC, New Orleans, LA
*Purpose: Adipose-derived stem cells (ASC) have increased levels of anti-inflammatory and immunoregulatory factors. Murine studies have shown stable macrochimerism and long-term full thickness skin graft…
2019 American Transplant Congress
Promotion of Chimerism by Lymphohematopoietic Graft-versus-Host Responses in Patients Receiving Intestinal Transplantation
J. Fu¹, J. Zuber¹, B. Shonts¹, A. Obradovic¹, K. Frangaj¹, E. E. Waffarn¹, S. Lau¹, T. M. Savage¹, S. Yang¹, K. Rogers¹, P. Satwani², S. Ho¹, Y. Shen³, A. Griesemer⁴, M. Martinez², T. Kato⁴, M. Sykes¹
¹Columbia Center for Translational Immunology, Department of Medicine, Columbia University, New York, NY, ²Department of Pediatrics, Columbia University, New York, NY, ³Department of Systems Biology, Columbia University, New York, NY, ⁴Department of Surgery, Columbia University, New York, NY
*Purpose: Long-term multilineage chimerism often appears in blood following intestinal transplantation (ITx), usually without graft-versus-host disease (GVHD). Patients with donor T cell macrochimerism (≥4%) in…
2018 American Transplant Congress
Donor Bone Marrow CXCR4+ Foxp3+ Treg Cells Are Essential for Costimulation Blockade-Induced Long-Term Survival of Murine Limb Transplants
L. Wang,¹ Z. Wang,¹ R. Han,¹ G. Ge,¹ L. Levin,² M. Levine,² W. Hancock.^1,2
¹CHOP, Philadelphia; ²UPenn, Philadelphia.
Vascularized composite allotransplantation (VCA) would likely be more widely performed if maintenance immunosuppression was not essential for graft acceptance. Accordingly, we used murine models of…
2018 American Transplant Congress
Pilot Study of Plasma Cell Niche-Targeted Therapy for Enhancement of Proteasome Inhibitor Effectiveness
S. Tremblay,¹ C. Castro-Rojas,² J. Driscoll,¹ S. Knechtle,³ A. Shields,¹ R. Alloway,¹ H. Singh,² D. Hildeman,² E. Woodle.¹
¹UCincinnati, Cincinnati; ²Cincinnati Children's, Cincinnati; ³Duke, Durham.
HLA antibody (Ab)-secreting bone marrow (BM) plasma cell (PC) survival depends on niche occupancy via a CXCR4 (PC)-CXCL12 (BM stromal cell) interaction. Plerixafor inhibits CXCR4-CXCL12…
2018 American Transplant Congress
Comparative Analysis of Bone Marrow versus Blood Monocyte-Derived Regulatory Dendritic Cells for Evaluation in a Miniature Swine Vascular Composite Allotransplantation Model
T. Elgendy,¹ D. Kim,² A. Zahorchak,¹ M. Ezzelarab,¹ M. Solari,² A. Thomson.¹
¹Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA; ²Department of Plastic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA.
Background: Dendritic cell therapy is a promising approach to reduced dependence on immunosuppressive drugs and for the promotion of tolerance in transplant recipients. Our aim…
2018 American Transplant Congress
The Ratio of CTLA4 to Perforin and Granzyme B as a Potential Biomarker of Tolerance in Chimeric Renal Transplant Patients
A. Khalil,¹ A. Chhabra,¹ A. Merchak,¹ L. Kahn,¹ J. Leventhal,² S. Ildstad.¹
¹Institute for Cellular Therapeutics, University of Louisville, Louisville, KY; ²Northwestern University, Chicago, IL.
Background: Thirty-six subjects have been enrolled in a phase II clinical trial aimed to induce tolerance through chimerism. Each subject received FCRx (mPBMC enriched in…
2018 American Transplant Congress
In Vivo Administration of Plerixafor and Bortezomib Results in Bone Marrow Plasma Cell Mobilization and Death While Inducing Biphasic Plasma Cell Apoptosis in Peripheral Blood
E. Woodle,¹ S. Tremblay,¹ C. Castro-Rojas,² S. Knechtle,³ A. Shields,¹ R. Alloway,¹ H. Singh,² J. Driscoll,¹ D. Hildeman.²
¹U Cincinnati, Cincinnati; ²Cincinnati Children's, Cincinnati; ³Duke, Durham.
Bone marrow (BM) plasma cells (PC) secreting HLA antibody (Ab) remain a persistent reservoir of donor specific Ab and present a significant hurdle to allograft…
2018 American Transplant Congress
Long Term Culture of Functional Bone Marrow-Derived Human Plasma Cells
N. Moore, K. Bonner, M. D'Costa, M. Stegall.
William J. von Liebig Center for Transplantation and Clinical Regeneration, Mayo Clinic, Rochester, MN.
Introduction Antibody-secreting plasma cells (PCs) are critically important for host defense, but also can cause antibody-mediated allograft rejection. The ability to study these cells is…