Articles tagged "Antigen presentation"

2020 American Transplant Congress
Lymph Node Stromal Laminin Alpha5 Regulates Antigen Specific T Cell Adaptive Immunity
L. Li¹, M. Shirkey¹, W. Piao¹, Y. Xiong¹, V. Saxena¹, T. Zhang¹, C. Paluskievicz¹, J. Zhao², R. Abdi², J. S. Bromberg¹
¹UMB, Baltimore, MD, ²Harvard University, Boston, MA
*Purpose: Laminin α5 (Lama5) is associated with immunity and regulates T cell entry and responses within the lymph node (LN). We hypothesized that stromal Lama5…
2020 American Transplant Congress
Role of Cross-Dressing in Kidney Transplantation
D. Zhao, R. Tieu, K. Abou-Daya, A. Williams, M. Oberbarnscheidt, F. Lakkis
Thomas E. Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: Activation of host T cells that mediate allograft rejection is relied on dendritic cells (DCs) presenting alloantigens to T cells. Our recent study revealed…
2020 American Transplant Congress
Migration of Passenger Leukocytes During Ex Vivo Warm Perfusion
L. Brasile¹, B. Stubenitsky²
¹Research and Development, BREONICS Inc., Watervliet, NY, ²Plastic, Reconstructive & Aesthetic Surgery, St. Antonius Hospital, Utrecht, Netherlands
*Purpose: Organ transplantation is a unique immunologic situation in which donor and recipient antigen presenting cells (APCs) are present upon reperfusion. Passenger leukocytes (PL) are…
2020 American Transplant Congress
Long-Term Allograft Survival via Donor MHC Chimerism Induced by Exosomes
B. Gonzalez Nolasco, X. Wang, M. Wang, A. Prunevieille, K. M. Lee, J. F. Markmann, G. Benichou
Surgery - Center for Transplantation Sciences, Massachusetts General Hospital & Harvard Medical School, Boston, MA
*Purpose: Allogeneic transplant tolerance has been achieved by inducing mixed hematopoietic chimerism via donor bone marrow transplantation. Tolerance mechanisms in this model involve the presentation…
2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
T. M. Coe¹, N. Serifis¹, S. Raigani¹, C. Carroll¹, C. G. Rickert¹, R. Matheson¹, D. Detelich², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, A. Azimzadeh¹, J. F. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Surgery, Tufts Medical Center, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…
2020 American Transplant Congress
The Role of Galectin-1 and Basement Membrane Remodeling in Kidney Antibody-Mediated Rejection
S. Clotet-Freixas¹, C. McEvoy¹, I. Batruch², M. Kotlyar³, C. Pastrello³, J. Van¹, M. Arambewela¹, A. Boshart¹, S. Farkona¹, A. Bozovic⁴, V. Kulasingam⁴, P. Chen¹, I. Jurisica³, A. Chruscinski¹, R. John⁴, A. Konvalinka⁵
¹Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada, ²Department of Laboratory Medicine and Pathobiology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada, ³Krembil Research Institute, University Health Network, Toronto, ON, Canada, ⁴Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, ⁵Department of Medicine, Division of Nephrology, University Health Network, Toronto, ON, Canada
*Purpose: Antibody-mediated rejection (AMR), the leading cause of kidney graft loss, is caused by anti-HLA and non-HLA antibodies against proteins in two main kidney compartments:…
2020 American Transplant Congress
Single Cell RNA Sequencing of Murine Kidney Allografts Identifies Macrophage Gene Signature Associated with Acute Rejection
A. Dangi¹, N. Natesh², M. Monal¹, J. Wang³, X. Shen², X. Luo¹
¹Nephrology/Medicine, Duke University, Durham, NC, ²Biomedical Engineering, Duke University, Durham, NC, ³Surgery, Duke University, Durham, NC
*Purpose: Evaluating kidney allografts using single cell RNA sequencing (scRNAseq) can provide a comprehensive qualitative and quantitative gene expression data on thousands of individual cells.…
2020 American Transplant Congress
Withaferin A Prolongs Islet Allograft Survival in a Mouse Transplant Model by Preventing T Lymphocyte Proliferation and Dendritic Cell Maturation
K. Kumano¹, M. A. Kanak², S. Vasu¹, Y. Liu¹, M. Lawrence¹, B. Naziruddin¹
¹Baylor University Medical Ctr, Dallas, TX, ²Virginial Commonwealth University Medical Center, Richmond, VA
*Purpose: Chronic decline in islet graft function and toxicities imposed by immunosuppressant drugs remain as challenges to the widespread use of islet transplantation for type…
2020 American Transplant Congress
Pleural Exosome Mediated Donor Antigen Trafficking Into Mediastinal Lymph Nodes Represents a Novel Pathway of Recipient T Cell Activation in Lung Transplantation
A. Habertheuer¹, A. Japp², C. Ram³, S. Chatterjee⁴, S. Rostami², L. Korutla⁵, T. Ochiya⁶, A. Naji², D. Kreisel⁷, P. Vallabhajosyula⁵
¹Surgery, University of Pittsburgh, Pittsburgh, PA, ²Surgery, University of Pennsylvania, Philadelphia, PA, ³New York University, New York, NY, ⁴Pathology, University of Pennsylvania, Philadelphia, PA, ⁵Surgery, Yale University, New Haven, CT, ⁶National Cancer Center Japan, Tokyo, Japan, ⁷Surgery, Washington University, St. Louis, MO
*Purpose: Studies in lung transplant models suggest that reestablishment of lymphatic continuum of pulmonary allograft via lymphangiogenesis is required for selective trafficking of donor antigen…
2020 American Transplant Congress
IL-10 Exposure Enhances IRAK-M Expression to Promote Endotoxin Tolerance and the T Cell Tolerogenic Potential of Liver Conventional DCs
Y. Zheng, R. Nakano, A. Thomson
Surgery, Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: Liver conventional myeloid (m)DC contribute to liver transplant tolerance. When freshly-isolated, they produce low levels of IL-12 but high levels of IL-10 compared with…