Articles tagged "Alloantibodies"

2018 American Transplant Congress
Presence of Donor Specific Antibodies is Associated with Accelerated Podocyte Detachment
A. Naik, J. Aqeel, D. Cibrik, M. Samaniego, S. Wang, M. Chowdhury, F. Afshinnia, R. Wiggins.
University of Michigan, Ann Arbor; University of Kansas, Kansas City.
Introduction: Both indication and 10-yr surveillance biopsy data point to the important role of progressive glomerular disease in intermediate and long-term allograft outcomes. As all…
2018 American Transplant Congress
A Novel Endothelial Cell Based Complement Dependent Cytotoxicity Test in Kidney Transplantation
R. Lammerts,¹ J. van den Born,¹ W. Dam,¹ M. Seelen,¹ B. Hepkema,² B. Kroesen,² M. Daha,¹ R. Pol,³ S. Berger,¹ On behalf of the COMBAT Consortium.¹
¹Department of Nephrology, University Medical Centre Groningen, Groningen, Netherlands; ²Department of Immunology, University Medical Centre Groningen, Groningen, Netherlands; ³Department of Surgery, University Medical Centre Groningen, Groningen, Netherlands.
Introduction:Relevant alloantibodies in kidney transplantation comprise anti-HLA antibodies, blood group antibodies (ABO incompatible) and anti-endothelial cell antibodies (AECA) and can initiate antibody-mediated rejection (ABMR). ABMR…
2018 American Transplant Congress
Long Term Culture of Functional Bone Marrow-Derived Human Plasma Cells
N. Moore, K. Bonner, M. D'Costa, M. Stegall.
William J. von Liebig Center for Transplantation and Clinical Regeneration, Mayo Clinic, Rochester, MN.
Introduction Antibody-secreting plasma cells (PCs) are critically important for host defense, but also can cause antibody-mediated allograft rejection. The ability to study these cells is…
2018 American Transplant Congress
Designing Novel Selective Immunoproteasome Inhibitors Reveals a Critical Role of the Immunoproteasome in Protecting Antibody Secreting B Cells from Apoptosis
J. Assaker,¹ N. Murakami,¹ S. Eskandari,¹ M. Uehara,¹ C. Nathan,² H. Li,³ G. Lin,² J. Azzi.¹
¹Transplantation Renal Center, Harvard Medical School, Boston; ²Microbiology and Immunology, Weill Cornell Medicine, New York; ³Cryo-EM Structural Biology Laboratory, Van Andel Research Institute, Grand Rapids.
Constitutive proteasomes (c-20S) are ubiquitously-expressed cellular proteases that degrade polyubiquitinated proteins and regulate cell functions. Its inhibitor, bortezomib, is a plasma cell-targeted therapy used in…
2018 American Transplant Congress
All DQ DSA May Not Be Equal: Comparing Outcomes between De Novo DQ DSA Specificities
M. Everly,¹ K. Briley,² A. Nguyen,¹ V. Jucaud,¹ A. Maldonado,³ C. Haisch,² P. Bolin,² S. Kendrick,⁴ H. Jones,⁴ K. McLawhorn,⁴ D. Leeser,² L. Rebellato.²
¹Terasaki Research Institute, Los Angeles; ²East Carolina University, Greenville, NC; ³Vidant Medical Center, Greenville, NC; ⁴Eastern Nephrology Associates, Greenville, NC.
It is not known if development of de novo DSA (dnDSA) to different HLA-DQ mismatches (serological) are associated with equal risk to a patient's allograft…
2018 American Transplant Congress
Involvement of PD-L1 Upregulation in Anti-A/B Antibody-Induced Graft Accommodation: Reduction of CD4 T Cell Allo-Response against Graft HLA Class II DR
K. Iwasaki,¹ Y. Miwa,¹ H. Hamana,³ H. Kishi,³ A. Muraguchi,³ K. Uchida,¹ T. Kobayashi.²
¹Kidney Disease and Transplant Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ²Renal Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; ³Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
[Background] Antibodies against transplanted organs cause complement-independent graft injury, but there remain many unknown aspects of its mechanism, in particular its effect on allo-response to…
2018 American Transplant Congress
BLyS^-/- Rats to Prevent Alloantibody Production
N. Bath,¹ B. Verhoven,¹ N. Wilson,¹ S. Reese,² L. Coons,¹ S. Panzer,² A. Djamali,² R. Redfield.¹
¹Surgery, University of Wisconsin, Madison, WI; ²Medicine, University of Wisconsin, Madison, WI.
Introduction: Antibody mediated rejection (AMR) is a major cause of kidney allograft failure. APRIL (A Proliferation Inducing Ligand) and BLyS (B Lymphocyte Stimulator) are two…
2018 American Transplant Congress
Approaches to Prevention of Antibody Rebound after HLA Sensitization in a Mouse Model
I. Kim, G. Wu, N-.N. Chai, A. Klein, S. Jordan.
Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA.
Purpose: Control of donor specific antibodies (DSA) is critical for prevention of antibody mediated rejection (ABMR). Removal of DSAs with PLEX often results in significant…
2018 American Transplant Congress
Follow Up of Patients Treated with the IgG Endopeptidase (IdeS) for Desensitization and HLA Incompatible (HLAi) Kidney Transplantation
S. Jordan,¹ J. Choi,¹ E. Huang,¹ R. Najjar,¹ A. Peng,¹ X. Zhang,¹ S. Louie,¹ M. Haas,² S. Sethi,¹ I. Kim,¹ A. Vo.¹
¹Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA; ²Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA.
Introduction:Donor-specific antibodies (DSAs) create an immunologic barrier to transplantation. This prevents transplantation for a significant percentage of highly-HLA sensitized patients (HS). Current desensitization therapies are…
2018 American Transplant Congress
A New Reality: Virtual Crossmatch Can Be Used Successfully as Pre-Transplant Compatibility Testing for Most Deceased Donor Transplants at a High Volume Center
R. Rajalingam,¹ D. Gae,¹ Z. Laszik,² G. Roll,³ M. Tavakol,³ C. Freise,³ D. Adey,⁴ A. Webber,⁴ D. Dobi,² K. Cunniffe,¹ G. da Gente,¹ N. Ascher,³ P. Stock,³ J. Roberts.³
¹Immunogenetics and Transplantation Laboratory, Dept. of Surgery, UCSF, San Francisco; ²Dept. of Pathology, UCSF, San Francisco; ³Dept. of Surgery, UCSF, San Francisco; ⁴Dept. of Medicine, UCSF, San Francisco.
Aim: We developed a reliable virtual crossmatch (VXM) method for use as pre-transplant XM for deceased donor (DD) kidney transplantation (KTx). Here we report the…