*Purpose: Uterus transplantation is a new treatment option for females with uterine factor infertility and a rapidly evolving field of organ transplantation. Proof-of-concept, i.e., birth of a child, has been achieved using grafts from both deceased and living donors. We aim to share our experience in performing the living-donor uterine procurement for uterus transplantation.

*Methods: Over a 2-year period we have performed 14 uterus transplantations at our institution using grafts from both deceased (n=2) and living donors (n=12). We used live action footage from surgery together with comprehensive illustrations, to demonstrate stepwise our technique of uterine graft procurement from a living donor. The main outcome measurements were surgery time and suitability of dissected specimen, donor complications, hospital stay and recovery.

*Results: This video article describes the vital steps in the uterine transplant process, including selecting an appropriate living uterus donor to achieve a successful uterus transplant outcome. The video outlines the key steps in a uterine donation procedure and shows footage from an actual uterus procurement. These steps include identifying and isolating the relevant vascular structures; uterine arteries and veins and utero-gonadal veins and retrieving them together with the uterus and part of the vagina. Once this has been completed, the organ is assessed and flushed on the backtable.

*Conclusions: Uterine transplantation is the first true treatment for uterine factor infertility. Most of the uterus transplants worldwide have been done with a uterine graft from a living donor. The living donor model has proven to be a safe procedure with little risk of donor complications and a good transplant outcome.

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