Introduction: Small, poor compliant or unstable bladders are a major problem in patients waiting for renal transplantation (RT). The experimental technique aims to develop and evaluate a new ileobladder model.

Methods: 15 rats (250-300gr) and 5 pigs (approximately 100kg) were used. General anesthetic was given and laparotomy was done through midline incision. Ileal loops were prepared for ileobladder. After cystectomy (down to 0.5 cm above trigon in rats, down to 1cm above trigon in pigs), anastomoses were done between antimesenteric sides of ileal loops and the remnant of the bladder with 6/0 prolene suture. The same procedure was performed simultaneously with RT in 3 other pigs

Results: 1 rat died on the first day due to hemorrhage. 2 rats survived for 5 days, 3 rats for 7 days, 3 rats for 11 days, and six rats were sacrificed for pathological evaluation after 3 months. 1 pig survived for 22 days, 1 for 9 days. Of the 3 pigs that received a simultaneous RT and 1 pig was sacrificed for pathological evaluation after 3 months. The others are still alive and doing well 80 and 72 days after surgery, respectively. Urinary discharge was normal in all of them. When ileobadder was opened, complete recovery of the anastomosis line was observed, and a normal healing process with moderate inflammation was reported and the muscular wall of the intestine of all of them showed hypertrophy that nearly reached the size of the bladder muscularis propria

Conclusions: Although some complications were faced as no draining procedure was used, in terms of technique, our new ileobladder model is promising for providing functional bladder volume. In the future, this technique can be useful for small bladder and bladder physiology disorders.

CITATION INFORMATION: Haberal M., Kirnap M., Ersoz D., Bacanli D., Ersoy Z., Bayzakov M., Torgay A., Ozdemir H. The Technique of Ileobladder and Kidney Transplantation in Rats and Pigs Am J Transplant. 2017;17 (suppl 3).

« Back to 2018 American Transplant Congress