The Summarized Assessment of Endothelin a Receptors Expression in Renal Transplant Compartments is Associated with Antibody Mediated Rejection
1Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Wroclaw, Poland, 2Department of Pathomorphology and Oncological Cytology, Wroclaw Medical University, Wroclaw, Poland, 3Research laboratory, Wroclaw Medical University, Wroclaw Medical University, Wroclaw, Poland, 4Department of Public Health, Wroclaw Medical University, Wroclaw, Poland, 5Department of General, Vascular and Transplant Surgery, Wroclaw Medical University, Wroclaw, Poland
Meeting: 2021 American Transplant Congress
Abstract number: 1042
Keywords: Antibodies, Endothelin, Rejection, Renal dysfunction
Topic: Clinical Science » Kidney » Kidney Chronic Antibody Mediated Rejection
Session Information
Session Name: Kidney Chronic Antibody Mediated Rejection
Session Type: Poster Abstract
Session Date & Time: None. Available on demand.
Location: Virtual
*Purpose: The presence of non-HLA anti-endothelin A receptor antibodies may be important in transplant injury but the expression of the endothelin A receptor (ETA receptor) in renal compartments has not been described yet. We assessed the presence and relevance of the ETA receptor in renal transplant biopsy for cause. The aim of our study was to evaluate the expression of ETA receptor in patients who had a transplant biopsy due to the deterioration of function.
*Methods: Expression of ETA receptors was analyzed in renal transplant biopsies using immunohistochemical method. Microscopic assessment of ETA receptors expression (rabbit polyclonal antibody, dilution: 1:100, catalog number: G094 (P25101); Assay Biotechnology Company, USA) was performed on 4 µm-thick paraffin sections, which were fitted on silanized slides (DAKO, Denmark). ETA receptors expression was analyzed in four compartments of renal transplant biopsies: glomeruli, vessels, tubular epithelium and interstitium. The assessment was presented using three-step scale (0: lack of expression, 1: mild to moderate immunoreactivity, 2: high expression). The results of each compartment for single biopsy were summarized and assessed in context of antibody mediated rejection.
*Results: Results: We analyzed 154 patients who had a renal allograft biopsy after renal transplantation. We noticed that patients with antibody mediated rejection had significantly higher summarized expression of ETA receptors 3,28 ± 1,56 in comparison to patients who had biopsy for other reason 1,47 ± 1,35. (p<0.000001).
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*Conclusions: The expression of endothelin A receptors in renal transplant compartments may be associated with antibody mediated rejection. The positive ETA receptor staining might be an important feature in the diagnosis of damage in progress of antibody mediated rejection. The identification of an injury with ETA receptor during rejection may help to the development of new treatment strategies to improve transplant survival.}$$
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To cite this abstract in AMA style:
Banasik M, Nowanska K, Donizy P, Koscielska-Kasprzak K, Zmonarski S, Kuriata-Kordek M, Tukiendorf A, Hałoń A, Janczak D, Krajewska M. The Summarized Assessment of Endothelin a Receptors Expression in Renal Transplant Compartments is Associated with Antibody Mediated Rejection [abstract]. Am J Transplant. 2021; 21 (suppl 3). https://atcmeetingabstracts.com/abstract/the-summarized-assessment-of-endothelin-a-receptors-expression-in-renal-transplant-compartments-is-associated-with-antibody-mediated-rejection/. Accessed May 11, 2025.« Back to 2021 American Transplant Congress