ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Membrane Attack Complex as a More Sensitive Tool to Evaluate Complement Activation in Kidney Transplant Recipients with Donor-Specific Antibodies

C. Arias-Cabrales, D. Redondo-Pachón, E. Rodríguez, M. Riera, A. Buxeda, M. Pérez-Sáez, C. Burballa, L. Llinas, J. Pascual, M. Crespo

Nephrology, Hospital del Mar, Barcelona, Spain

Meeting: 2022 American Transplant Congress

Abstract number: 1412

Keywords: Antibodies, Kidney, N/A, Outcome

Topic: Clinical Science » Kidney » 45 - Kidney Chronic Antibody Mediated Rejection

Session Information

Session Name: Kidney Chronic Antibody Mediated Rejection

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

*Purpose: Kidney transplant recipients with late HLA donor-specific antibodies (DSA) detected with single antigen beads (SAB) exhibit more frequent antibody-mediated rejection (ABMR) and graft loss. Complement activation is one of the mechanisms involved in ABMR damage and has been assessed by the ability of SAB to bind C1q or C3d. The aim of our study was to evaluate the comparative prognostic information provided by MAC serum level in patients with late DSA class II.

*Methods: Of 425 kidney transplant patients (KT) monitored for HLA DSA 2008-2012 and prospectively followed, we selected those with HLA II DSA (DSA-II) and a contemporaneous control group of patients without DSA and a kidney biopsy without ABMR. We quantified serum levels of membrane attack complex (MACs) by ELISA in both groups before transplantation and at the moment of post-transplant SAB monitoring. Then, we analyzed the relationship between MAC and the ability of DSA-II to bind C1q/C3d as well as their MFI, and their possible prognostic impact.

*Results: 43 KT patients showed DSA-II and presented poor graft survival compared with DSA– patients. MAC levels at the moment of DSA were higher than in the same patients pre-transplant and post-transplantation before DSA (Figure 1). 32 DSA+ cases were C3d+ and 28 C1q+. In DSA+ patients, neither C3d+, nor C1q+, MFI over 2995 or MAC level were related with poor death-censored graft survival, but all of these were related to lower ABMR free survival (Figure 2). MAC level was the most sensitive assay and showed the best negative predictive value to discriminate ABMR (S=91% and VPN=66%) compared with C3d, C1q, and MFI>2995

*Conclusions: Late DSA class II are related with worse graft survival and a high rate of ABMR (55%). Their capacity to activate complement assessed by C3d binding assays or MACs level are related with ABMR diagnosis, been MACs level determination a more sensitive marker than C3d and C1q.

  • Tweet
  • Click to email a link to a friend (Opens in new window) Email
  • Click to print (Opens in new window) Print

To cite this abstract in AMA style:

Arias-Cabrales C, Redondo-Pachón D, Rodríguez E, Riera M, Buxeda A, Pérez-Sáez M, Burballa C, Llinas L, Pascual J, Crespo M. Membrane Attack Complex as a More Sensitive Tool to Evaluate Complement Activation in Kidney Transplant Recipients with Donor-Specific Antibodies [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/membrane-attack-complex-as-a-more-sensitive-tool-to-evaluate-complement-activation-in-kidney-transplant-recipients-with-donor-specific-antibodies/. Accessed May 30, 2025.

« Back to 2022 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences