Antecedents: Liver transplantation remains the sole therapy for a certain number of liver diseases at end stage. Liver proteolysis that occurs during cold storage influences the subsequent graft outcome. Data evaluating the incidence of preservation solution on liver proteolysis degree are poor. In this communication, we investigate the effect of IGL- 1 preservation solution on steatotic liver proteolysis when compared to UW one. Experimental: Steatotic livers were preserved in UW and IGL-1 preservation solutions for 24h at 4ºC, respectively and then subjected to “ex vivo” perfusion (2h; 37ºC). To asses liver proteolysis, the amino-acids content in tissue specimens were measured by reverse-phase high performance liquid chromatography (HPLC). Total free amino release was correlated with liver injury (transaminases and high mobility group box (HGMB) release) and function (bile output, vascular resistance and bromosulfophthalein clearance). In addition, oxidative stress, mitochondrial damage and apoptosis degree were evaluated. Results: Amino acids (alanine, prolyne, leucine, isoleucine, methionine, lysine, ornithine, and threonine among others) levels were significantly lower in grafts preserved in IGL-1 solution than UW one (p < 0.05). This was consistent with a significant diminution of HMGB1 protein levels and liver injury (AST/ALT), as well as an ameliorated function. Also, the oxidative (MDA) stress, mitochondrial damage (GLDH) and apoptosis (GSK-3beta and VDAC) were efficiently prevented by the use of IGL- 1 solution Conclusion: IGL-1 solution prevented efficiently fatty liver proteolysis vs UW; confirming thus a better liver graft preservation.

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