Introduction

Gut microbiota modifications (dysbiosis) have been observed in obese and/or diabetic patients. Kidney transplant recipients (KTR) are particularly exposed to the development of metabolic disorders, both before and after kidney transplantation (KT). Here, we investigated gut microbiota composition in KTR with or without metabolic disorders.

Methods

Feces were collected from KTR with or without metabolic disorders before and 3-12 months after KT. Fecal bacterial DNA was extracted, and qPCRs were performed to quantify 9 bacterial species or groups. Results were expressed as relative proportions of total bacteria.

Results

52 KTR were included: median age was 56 years, 35% were women. Before KT, there were 29% of diabetic patients (called “diabetic-pre”) and 15% of obese patients. After KT, 27% of patients developed New Onset Diabetes (“NODAT”) and 5% developed obesity. Before KT, the Firmicutes/Bacteroidetes ratio was increased in the “diabetic-pre” group, and Faecalibacterium prausnitzii relative quantity was decreased in the “diabetic-pre” and in the NODAT groups, compared to controls. After transplantation, Lactobacillus relative proportion was increased, and Akkermansia muciniphila relative quantity was decreased in diabetic and obese patients, compared to controls.

Conclusion

Gut dysbiosis is associated with metabolic disorders in KTR. This work evidences F. prausnitzii as a possible predictive marker for NODAT and A. muciniphila as a possible marker of metabolic disease.

CITATION INFORMATION: Lecronier M, Tashk P, Aron-Wisnewsky J, Denamur E, Tenaillon O, Barrou B, Tourret J. Gut Dysbiosis and Metabolic Disorders in Kidney Transplant Recipients. Am J Transplant. 2016;16 (suppl 3).

« Back to 2016 American Transplant Congress