ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2022 American Transplant Congress
    • 2021 American Transplant Congress
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2021 Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Search

Differential Regulation of Regulatory T Cells and Th17 Cells by mTOR Inhibitor in Kidney Transplant Recipients

K. Kim, B. Chung, B. Kim, M. Cho, C. Yang

Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
Transplant Research Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
Division of Nephrology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea

Meeting: 2013 American Transplant Congress

Abstract number: B1025

Background : Accumulating evidence suggests that Treg/Th17 axis have important role in long term allograft survival in kidney transplantation. The aim of this study is to compare the effect between tacrolimus (Tac) and sirolimus (SRL) in the regulation of Treg/Th17 axis.

Methods: To investigate the effect of SRL or Tac on Treg/Th17 axis, Th1 (IFN-g), Th2 (IL-4), Th17 (IL-17) and Treg (Foxp3+) were quantitatively determined upon T-cell-specific stimulation in blood mononuclear cells (PBMCs) from healthy individuals (n = 11; in vitro) and kidney-transplant patients (n = 5; ex vivo) under clinically relevant concentrations of SRL or Tac. In addition, we analyzed the changes in T cell subsets in peripheral blood mononuclear cells (PBMCs) using FACs analysis, mRNA levels using real-time PCR and signaling molecules using western blotting. In vivo, we investigated the change of T cell subset after conversion from Tac to SRL.

Results: In vitro, Tac suppressed Th1, Th2 and Treg cells in a concentration-dependent manner, but did not suppress Th17 cells even at high concentration. During therapy with SRL, without CNI, the Th1, Th2 and Th17 production was decreased, but the Treg was not decreased, in comparison to treatment with Tac. IL-17 mRNA levels were significantly decrease in treatment with SRL and CNI as compared with the Th17 condition. However, Foxp3 mRNA expression levels were significantly increased in treatment with SRL. Kidney Transplant recipients who took SRL showed decreased production of Th17 and Th2 cytokines in PBMC, compared to treatment with Tac.

Conclusion: This study suggests that SRL is superior in the regulation of Th17/Treg axis to Treg favorable condition compared to Tac., which may give benefit in long term allograft survival.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Kim K, Chung B, Kim B, Cho M, Yang C. Differential Regulation of Regulatory T Cells and Th17 Cells by mTOR Inhibitor in Kidney Transplant Recipients [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/differential-regulation-of-regulatory-t-cells-and-th17-cells-by-mtor-inhibitor-in-kidney-transplant-recipients/. Accessed May 14, 2025.

« Back to 2013 American Transplant Congress

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2025 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy | Terms of Use | Cookie Preferences