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c-Kit+ Cells Are Kidney-Specific Progenitor/Stem Cells with Regenerative Potential.

S. Abreu Gomes,1 G. Goss,2 B. Goldstein,2 B. Seidler,3 D. Saur,3 J. Hare,2 E. Bevilaqua Rangel.1

1Albert Einstein Hospital, Sao Paulo, SP, Brazil
2University of Miami, Miami
3Medizinische Klinik, Technische Universität München, Munich, Germany.

Meeting: 2016 American Transplant Congress

Abstract number: D34

Keywords: Kidney, Stem cells

Session Information

Session Name: Poster Session D: Chimerism/Stem Cells, Cellular/Islet Transplantation, Innate Immunity, Chronic Rejection

Session Type: Poster Session

Date: Tuesday, June 14, 2016

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Introduction: Identification of progenitor/stem cell populations in mammalian tissues is important for therapeutic applications and for understanding biological processes. We recently reported that c-Kit+ cells isolated from developing rat kidneys exhibit progenitor/stem cell properties. We hypothesize therefore that c-Kit+ cells represent a tissue-specific progenitor/stem cell population that is involved in kidney development, is maintained during adult life, and contributes to kidney regeneration. Methods: For lineage tracing, we crossed the inducible c-Kit Cre reporter mice with IRG, mT;mG, R26R LacZ, and multicolored Confetti mice. By varying the timing of tamoxifen treatment, c-Kit+ cells and their descendants were specifically labelled with enhanced green fluorescent protein (EGFP), LacZ or multicolor, and their spatiotemporal distribution was followed during kidney development and acute kidney injury (ischemia-reperfusion and rhabdomyolysis). Results: c-Kit expression was more abundant in early post-natal (P) period (7.91 in P0.5-3.5; 10.6 in P7-14 vs 3.13 in embryonic [E]17.5-18.5, P<0.0001), and was maintained through adult life, although at lower levels (5.7 in P30 and 2.2 in P90-180). When tamoxifen was administered during E7.5-9.5, a few EGFP/LacZ+ cells were observed in tubular segments from cortex to medulla, and at E10.5-12.5, when ureteric bud invades the metanephric mesenchyma, ribbons of c-Kit-EGFP/LacZ+ cells expanded to form tubular structures and were detected in structures resembling the S-shaped bodies. In post-natal period, the number of c-Kit-EGFP/LacZ+/clonal multicolored cells increased in the cortex, medulla, and papilla. In adult mice, c-Kit-EGFP/LacZ+/clonal multicolored cells were found in distinct renal segments (macula densa, distal tubules and collecting ducts). After acute kidney injury, the number of c-Kit+ clones increased from 10±3 to 36.5±8 (P<0.0001), notably in the outer medulla. Conclusions: c-Kit marks a kidney progenitor/stem cell population and may have therapeutic application.

CITATION INFORMATION: Abreu Gomes S, Goss G, Goldstein B, Seidler B, Saur D, Hare J, Bevilaqua Rangel E. c-Kit+ Cells Are Kidney-Specific Progenitor/Stem Cells with Regenerative Potential. Am J Transplant. 2016;16 (suppl 3).

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To cite this abstract in AMA style:

Gomes SAbreu, Goss G, Goldstein B, Seidler B, Saur D, Hare J, Rangel EBevilaqua. c-Kit+ Cells Are Kidney-Specific Progenitor/Stem Cells with Regenerative Potential. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/c-kit-cells-are-kidney-specific-progenitorstem-cells-with-regenerative-potential/. Accessed June 1, 2025.

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