Association of Interferon-γ Gene Polymorphisms with Cytomegalovirus Viremia in Renal Allograft Recipients
National Institute of Transplantation, Los Angeles, CA
St. Vincent Medical Center, Los Angeles, CA
Western University of Health Sciences, Pomona, CA
Roosevelt University College of Pharmacy, Schaumburg, IL
University of Southern California, Los Angeles, CA
Transplant Research Institute, Los Angeles, CA
Meeting: 2013 American Transplant Congress
Abstract number: 213
Background: Cytomegalovirus (CMV) is the most common cause of viral infection, causing morbidity and mortality among kidney transplant recipients (RTRs). Cytokines such as tumor necrosis factor- Α (TNF-Α), interleukin-10 (IL10), and interferon-Γ (IFN-Γ) have been shown to possess antiviral properties and their polymorphisms are associated with disease outcome. The aim was to investigate the association of gene polymorphisms in the IL10, IFN-Γ, TNF-Α, and toll-like receptor 2 (TLR2) with CMV viremia in RTRs.
Methods: IL10 (-1082 A>G, -592 A>C); TNF-Α -308 A>G; IFN-Γ +874 A>T; and TLR2- Arg753Gln G>A gene polymorphisms were studied in 237 RTRs (52 RTRs with CMV viremia and 185 without CMV viremia), using DNA-based polymerase chain reaction with sequence-specific primers and restriction. Stepwise logistic regression analysis was used to generate univariate and multivariate odd ratios to validate the significance of trends.
Results: Median time to CMV viremia was 6 months with a mean peak CMV viral load of 7925 copies per ml. Patients with donor-positive/recipient-negative [D+/R-] serostatus were found to be associated with a high risk of CMV viremia (p=0.001). A statistically significant correlation was found between IFN-Γ +874 A>T polymorphism and the risk of the CMV infection. The IFN-Γ +874 A allele (AA + AT) genotype was associated with a higher risk of CMV viremia (OR: 3.2, 95% CI: 1.2-8.5, p=0.01) while the high producer IFN-Γ+874 TT genotype was associated with a lower risk of CMV viremia (OR:0.31, 95%CI:0.11-0.83, p=0.015). The allelic as well as genotypic frequencies of TNF-Α, IL-10 and TLR2 did not significantly differ between CMV viremia and control group.
Conclusion: IFN-Γ gene polymorphisms could be an important risk factor for CMV infection, whereas the high producer IFN-Γ+874 TT genotype appears to be a marker for protection against CMV viremia by inhibiting the viral replication.
To cite this abstract in AMA style:
Vu D, Sakharkar P, Shah T, Qazi Y, Naraghi R, Poulsen J, Hutchinson I, Min D. Association of Interferon-γ Gene Polymorphisms with Cytomegalovirus Viremia in Renal Allograft Recipients [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/association-of-interferon-gene-polymorphisms-with-cytomegalovirus-viremia-in-renal-allograft-recipients/. Accessed November 22, 2024.« Back to 2013 American Transplant Congress