*Purpose: The impact of muscle injury from vascularized composite allograft (VCA) transplantation ischemia-reperfusion injury on renal function is not well known. The aim of this study was to evaluate for acute kidney injury in a small animal hind limb transplant (HLT) model.

*Methods: 300 g Male Brown-Norway rats underwent syngeneic HLT following either 6 or 24 hours of cold ischemia time (CIT). Recipients were euthanized on post-operative day (POD) 3 or 6. Sera was collected from donors after limb procurement and from recipients immediately prior to euthanasia. Non-parametric Kruskal-Wallis test was used to compare the groups.

*Results: Groups included donors (n = 5), 6 hour CIT/POD6 (n = 2), 24 hour CIT/POD3 (n = 3), and 24 hour CIT/POD6 (n = 5). ALT was significantly higher with 24 hour CIT on POD3 (p = 0.04, Figure 1). Donors also had evidence of muscle injury as signified by elevated CK and LDH. None of the groups had elevated creatinine and there was no inter-group differences in creatinine, BUN, or potassium (Figure 2).

*Conclusions: Muscle injury following VCA transplantation is transient and appears to resolve within a week in a small animal HLT model. We found no evidence of change in renal function in this model. Limb procurement also led to systemically elevated products of muscle injury in the donors, but this did not lead to acute renal injury.

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