Muscle Damage from Hind Limb Transplantation Does Not Lead to Acute Kidney Injury
University of Colorado Anschutz Medical, Aurora, CO
Meeting: 2022 American Transplant Congress
Abstract number: 959
Keywords: Kidney
Topic: Basic & Clinical Science » Basic & Clinical Science » 20 - VCA
Session Information
Session Time: 7:00pm-8:00pm
Presentation Time: 7:00pm-8:00pm
Location: Hynes Halls C & D
*Purpose: The impact of muscle injury from vascularized composite allograft (VCA) transplantation ischemia-reperfusion injury on renal function is not well known. The aim of this study was to evaluate for acute kidney injury in a small animal hind limb transplant (HLT) model.
*Methods: 300 g Male Brown-Norway rats underwent syngeneic HLT following either 6 or 24 hours of cold ischemia time (CIT). Recipients were euthanized on post-operative day (POD) 3 or 6. Sera was collected from donors after limb procurement and from recipients immediately prior to euthanasia. Non-parametric Kruskal-Wallis test was used to compare the groups.
*Results: Groups included donors (n = 5), 6 hour CIT/POD6 (n = 2), 24 hour CIT/POD3 (n = 3), and 24 hour CIT/POD6 (n = 5). ALT was significantly higher with 24 hour CIT on POD3 (p = 0.04, Figure 1). Donors also had evidence of muscle injury as signified by elevated CK and LDH. None of the groups had elevated creatinine and there was no inter-group differences in creatinine, BUN, or potassium (Figure 2).
*Conclusions: Muscle injury following VCA transplantation is transient and appears to resolve within a week in a small animal HLT model. We found no evidence of change in renal function in this model. Limb procurement also led to systemically elevated products of muscle injury in the donors, but this did not lead to acute renal injury.
To cite this abstract in AMA style:
Yoeli D, Wang Z, Huang J, Wang Y, Li B, Jain S, Nakra N, Rampalski K, Su A, Mathes D, Washington K, Farkash E, Huang C, Jani A. Muscle Damage from Hind Limb Transplantation Does Not Lead to Acute Kidney Injury [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/muscle-damage-from-hind-limb-transplantation-does-not-lead-to-acute-kidney-injury/. Accessed September 7, 2024.« Back to 2022 American Transplant Congress