Difference in Inflammatory Response between Clinical Allogeneic and Autologous Islet Transplantation

M. Takita, M. Kanak, F. Kunnathodi, R. Shahbazov, M. Lawrence, N. Onaca, B. Naziruddin, M. Levy

Islet Cell Laboratory, Baylor Research Institute, Dallas/Fort Worth, TX
Institute for Biomedical Studies, Baylor University, Waco, TX
Baylor Annette C. and Harold C. Simmons Transplant Institute, Dallas, TX

Meeting: 2013 American Transplant Congress

Abstract number: D1582

Background: Pancreatic islet transplantation (IT) can serve for patients with type 1 diabetes by allogeneic transplant as well as refractory chronic pancreatitis by autologous form. Recently TNFΑ inhibition with immunosuppression was reported to be a beneficial factor for islet survival in allogeneic IT; however, differences in inflammatory reaction has not been determined yet.

Methods: Five patients with autologous IT (Auto-IT group) and four with allogeneic IT (Allo-IT group) were included. Auto-IT patients had similar characteristics to Allo-IT group (Table). Both groups received TNFΑ inhibition (etanercept on day 0,1,4 and 7) and Allo-IT group was additionally administered IL-1Β recepter antagonist (100mg for day 0 to 7) with thymoglobulin immnosuppression induction. Inflammatory cytokines were evaluated with Luminex assay system using peri-transplant blood samples.

Table: Patient/Donor characteristics.
	Auto-IT group (n=5)	Allo-IT group (n=4)	p value
Patient age (year)	46±3	44±8	0.83
Patient Sex; Female, n(%)	4 (80%)	2 (50%)	0.52
Patient Body mass index (kg/m²)	25.9[pulsmn]1.7	22.7[pulsmn]0.7	0.12
Pancreas weight (g) ^†	84±9	111±14	0.17
Transplanted islet mass (×10³IEQ/kg of body weight)	8.7±1.3	9.1±0.5	0.82
Purity (%)	41±9	51±5	0.34
Transplanted tissue volume (mL)	12.7±2.2	9.5±0.2	0.22

Average±S.E. are shown. Student t or Fisher's exact tests were employed. ^†Donor pancreas weights for allo-IT group.

Results: Proinsulin levels in early post-transplant period, which suggest islet damage, were higher in Allo-IT than Auto-IT group (*p<0.05 for 1 hour-post IT samples, Figure). Inflammatory cytokines IP-10, IL-8 and TNFΑ in Allo-IT were significantly elevated compared to Auto-IT group after islet infusion.

Conclusions: Management of inflammation during early post-IT period is still critical to improve graft function in allogeneic IT even though TNFΑ-IL-1Β double blockage is employed when compared to autologous IT.

To cite this abstract in AMA style:

Takita M, Kanak M, Kunnathodi F, Shahbazov R, Lawrence M, Onaca N, Naziruddin B, Levy M. Difference in Inflammatory Response between Clinical Allogeneic and Autologous Islet Transplantation [abstract]. Am J Transplant. 2013; 13 (suppl 5). https://atcmeetingabstracts.com/abstract/difference-in-inflammatory-response-between-clinical-allogeneic-and-autologous-islet-transplantation/. Accessed May 17, 2025.

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