Purpose T-cell depletion by immunosuppressive antibodies has been increasingly used in treatment and prevention of allograft rejection. However, the impact of T-cell depletion on B-cell homeostasis remains poorly understood. This work evaluated the B-cell pool changes caused by anti T-cell antibodies.
Method C57BL/6 mice sensitized with skin allografts from a HLA.A2 transgenic mouse were T-cell depleted with intraperitoneal injections of anti-CD3e mAb. B lineage cell development in the central (bone marrow, BM) and peripheral (spleen) lymphoid organs were studied.
Results B cells expressing an immature phenotype (B220losIgM+ sIgD–) accumulate in the spleens following T-cell depletion by anti-CD3. The surge of immature B-cells in the spleen is anti-CD3 induced, but not alloantigen dependent. FACS analysis of the central (BM) and peripheral (spleen) B-cells harvested at 72 hours after T-cell depletion revealed that B220losIgM+ CD93+ pre-B-cell population in the BM were reduced while the B220losIgM+ CD23–CD93+/-sIgD– immature B-cells in the spleens were increased. Immunofluorescent microscopy confirmed B-cell proliferation and T-cell depletion in the spleen following anti-CD3 treatment. To further study the impact of anti-CD3 on BM T-cells, marrow cells were harvested from the femurs at day 3 after injection of anti-mouse CD3 mAb. Flow cytometric analysis demonstrated that (1) different subsets of T-cells existed in the naive BM; and (2) anti-CD3 significantly reduced T-cells, including CD8+, CD4+ and FoxP3+ Treg. Most importantly, T-cell depletion by anti-CD3 is associated with a reduction in B220+ cells in the BM and an increase B220lo cells in the spleens.
Conclusion Following systemic T-cell depletion, including BM T-cells the peripheral B-cell pool was repopulated with immature B-cells (B220loCD23–CD93+sIgM+sIgD–). The BM immature B-cell subset was significantly reduced, suggesting these BM immature B-cells have migrated into the periphery. The association of T-cell depletion to immature B-cells migration to the periphery indicates that T-cells may play a critical role in controlling release of immature B-cells from the BM to the periphery. Further studies to determine the mechanism by which T-cells regulate B-cell homeostasis would allow for designing new immunosuppression strategies to improve transplant survival.
To cite this abstract in AMA style:Wu G, Chai N, Jordan S, Klein A. T-Cell Depletion by Anti-CD3ε Is Associated with Dyshomeostasis in the Immature B-Cell Pool [abstract]. Am J Transplant. 2013; 13 (suppl 5). http://atcmeetingabstracts.com/abstract/t-cell-depletion-by-anti-cd3-is-associated-with-dyshomeostasis-in-the-immature-b-cell-pool/. Accessed September 21, 2017.
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