Human Leucocyte Antigen (HLA) matching is an important factor in long term graft survival in kidney transplantations. Natural Killer (NK) cells with their MHC recognizing Killer immunoglobulin like receptors (KIRs) are bridging cells between innate and adaptive immune response. In that context, NK response to graft maybe responsible in possible reactions seen short after transplantation. We aimed to evaluate matching status of KIRs between recipient and donor and KIR-HLA-C interactions and short term graft functions as well as their surface expression patterns before and after kidney transplantations.
Sixteen patients (8 female, 8 male, average age: 37 ± 12 years) were enrolled in the study. HLA-A,-B,-C, and DR tissue groups and KIR genotypes were determined from patients' and related donors DNA with PCR-SSP or PCR-SSO methods. HLA-Cs were determined by high resolution PCR-SSO typing. KIR phenotypes were examined by flow cytometry on peripheral blood NK cells isolated from patients prior and after 5-7th days of renal transplantation. KIR Haplotype analysis was performed by using analysis program in http://www.allelefrequencies.net.
There was no difference in short term graft function between the groups of KIR genotype matched and unmatched recipient-donor pairs.
Similarly, no difference was observed in comparison between the pairs who have matched or unmatched C1 and C2 groups in terms of heterozygosity or homozygosity. In the evaluation of KIR genotypes in accordance with HLA-C1/C2 groups, no significant difference in early graft function was determined.
Surface expressions of CD158f (2DL5), CD158e1 (3DL1), CD158a/h (2DL1/2DS1), CD158b (2DL2/2DL3) were investigated on recipients' NK cells before and after transplantation. Although a slight decrease in the expression of KIR3DL1 after transplantation was observed no correlation was determined with graft function.
We concluded that matching status of KIR haplotypes and interaction between KIR haplotype-HLA-C groups between donor-recipients pairs did not affect the early graft functions in renal transplantations.
To cite this abstract in AMA style:Eksioglu-Demiralp E, Elbasi M, Ture-Ozdemir F, Tulunay A, Tatli-Aydin I, Turkmen A, Kocak B. Killer Immunoglobulin-Like Receptor (KIR) Mismatches and KIR-HLA-C Interactions Do Not Affect Short Term Graft Function in Kidney Transplantation [abstract]. Am J Transplant. 2013; 13 (suppl 5). http://atcmeetingabstracts.com/abstract/killer-immunoglobulin-like-receptor-kir-mismatches-and-kir-hla-c-interactions-do-not-affect-short-term-graft-function-in-kidney-transplantation/. Accessed September 24, 2017.
« Back to 2013 American Transplant Congress